Nonreplicating intracellular bacterial vector for conjugative DNA transfer into mitochondria.

Pharmaceutical Research
Young Geol Yoon, Michael D Koob

Abstract

We have previously shown that DNA constructs can be introduced into isolated mitochondria through the process of conjugative transfer from an E. coli host. We set out to generate a conjugative E. coli strain that would be able to introduce itself into the cytoplasm of a mammalian cell for the purpose of transferring DNA into the mitochondria in the cell. We have now developed a method for making E. coli strains from which nonreplicating populations of daughter cells can be generated. We used this approach to modify a facultative intracellular enteroinvasive E. coli (EIEC) and introduced conjugative functions to this new strain. We demonstrate that this new strain can generate large populations of nonreplicating cells that are capable of conjugative transfer to other cells and can readily invade mammalian tissue culture cells, live in the cytoplasm of the cell for several days, and that do not kill the invaded mammalian cell. We successfully constructed an E. coli host suitable for intracellular conjugative transfer but, due to the lack of suitable mitochondrial screening or selectable markers, we have not yet been able to determine if these bacterial vectors can in fact transfer DNA into intracelluar mitochondria.

References

Aug 1, 1977·Journal of Bacteriology·T Hashimoto-Gotoh, M Sekiguchi
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Oct 12, 1990·Science·M Koob, W Szybalski
Apr 1, 1985·Infection and Immunity·E V OaksS B Formal
Jan 1, 1971·Molecular & General Genetics : MGG·J A Wechsler, J D Gross
Jan 1, 1983·Annual Review of Biochemistry·D J Waxman, J L Strominger
Nov 30, 1994·Annals of the New York Academy of Sciences·M D KoobD C Cameron
May 6, 1999·Frontiers in Bioscience : a Journal and Virtual Library·V L Waters
Dec 1, 2001·Nature Genetics·V L Waters
Sep 15, 2005·Nucleic Acids Research·Young Geol Yoon, Michael D Koob
Sep 27, 2005·FEMS Microbiology Letters·Claude Parsot
May 3, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Young Geol Yoon, Michael Duane Koob

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Citations

May 23, 2012·Mitochondrion·Adnan Khan NiaziAndré Dietrich
Jun 10, 2014·Mitochondrion·C B JacksonA Schaller

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