Nonribosomal peptides, key biocontrol components for Pseudomonas fluorescens In5, isolated from a Greenlandic suppressive soil

MBio
Charlotte Frydenlund MichelsenPeter Stougaard

Abstract

Potatoes are cultivated in southwest Greenland without the use of pesticides and with limited crop rotation. Despite the fact that plant-pathogenic fungi are present, no severe-disease outbreaks have yet been observed. In this report, we document that a potato soil at Inneruulalik in southern Greenland is suppressive against Rhizoctonia solani Ag3 and uncover the suppressive antifungal mechanism of a highly potent biocontrol bacterium, Pseudomonas fluorescens In5, isolated from the suppressive potato soil. A combination of molecular genetics, genomics, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) imaging mass spectrometry (IMS) revealed an antifungal genomic island in P. fluorescens In5 encoding two nonribosomal peptides, nunamycin and nunapeptin, which are key components for the biocontrol activity by strain In5 in vitro and in soil microcosm experiments. Furthermore, complex microbial behaviors were highlighted. Whereas nunamycin was demonstrated to inhibit the mycelial growth of R. solani Ag3, but not that of Pythium aphanidermatum, nunapeptin instead inhibited P. aphanidermatum but not R. solani Ag3. Moreover, the synthesis of nunamycin by P. fluorescens In5 was inhibited in the presence of P. ...Continue Reading

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Citations

Oct 12, 2016·The Journal of General and Applied Microbiology·Guillermo A MaronicheAlejandro Perticari
May 11, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ying-Ning HoYu-Liang Yang
Jan 13, 2018·Frontiers in Microbiology·Ruth Gómez ExpósitoJos M Raaijmakers
Oct 6, 2015·Journal of Industrial Microbiology & Biotechnology·Michael J SmanskiLinda L Kinkel
Aug 23, 2019·Natural Product Reports·Sebastian Götze, Pierre Stallforth
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Zachary L ReitzAlison Butler
Sep 5, 2020·Critical Reviews in Microbiology·Léa GirardRené De Mot
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May 16, 2019·Annual Review of Microbiology·Viviane CordovezJos M Raaijmakers
Mar 5, 2020·Microbiome·Wu XiongStefan Geisen
Jul 4, 2018·Biotechnology Letters·Yun XuePing Li
Jun 24, 2018·Archives of Microbiology·Adetomiwa Ayodele Adeniji, Olubukola Oluranti Babalola
Jun 8, 2021·Chemical Science·Zi Fei XuHui Ming Ge

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
gel filtration

Software Mentioned

FlexImaging
FlexControl
Compass
FlexAnalysis

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