PMID: 7539215May 1, 1995Paper

Nonselective cation channel activation during wound healing in the corneal endothelium

The American Journal of Physiology
M A Watsky

Abstract

Rabbit corneas were injured by mechanical or thermal trauma. At several time points after wounding, corneal endothelial cells were isolated and their ion channels examined using standard and amphotericin perforated-patch whole cell patch-clamp configurations. Within 15-24 h after mechanical or thermal trauma, a nonselective cation current was observed in 79% of the cells examined that was not present in unwounded or sham-wounded corneas. By 73 h postwounding, the current was present in only 10% of the cells examined. The wound healing-induced current is outwardly rectifying, activates at depolarized voltages, shows no sign of inactivation, and is inhibited by flufenamic acid, quinidine, and acetate. In addition to this new current, it was observed that endothelial cells from freeze-wounded corneas no longer expressed the transient K+ current seen in control, sham, and mechanically wounded corneas. Corneal endothelial superfusion experiments found no significant difference in swelling rates between control and flufenamic acid-superfused wounded corneas, indicating that the wound healing-induced channel is not involved in the stromal hydration maintenance function of the corneal endothelium.

References

Jun 1, 1992·The Journal of Membrane Biology·M A WatskyJ L Rae
Jun 11, 1992·Experimental Eye Research·M ChiangJ W Vanable
Mar 1, 1991·Journal of Neuroscience Methods·J RaeM Watsky
Sep 1, 1990·Current Eye Research·H K SoongS Bafna
Aug 1, 1988·Developmental Biology·A M RajnicekK R Robinson
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·E M EltingeJ W Vanable
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1984·Current Eye Research·D H GeroskiH F Edelhauser

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Citations

May 12, 1998·The American Journal of Physiology·K LiliomM A Watsky
May 18, 2005·American Journal of Physiology. Cell Physiology·Silvia ChiffletSilvina Grasso
Aug 9, 2003·The Journal of Biological Chemistry·Xinpo JiangLyanne C Schlichter
Feb 9, 2012·International Journal of Cell Biology·Silvia Chifflet, Julio A Hernández
Apr 25, 2014·Advances in Wound Care·Brian Reid, Min Zhao
Mar 27, 2010·Experimental Eye Research·Stefan MerglerUwe Pleyer

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