Nonsense-mediated decay enables intron gain in Drosophila.

PLoS Genetics
Ashley FarlowChristian Schlötterer

Abstract

Intron number varies considerably among genomes, but despite their fundamental importance, the mutational mechanisms and evolutionary processes underlying the expansion of intron number remain unknown. Here we show that Drosophila, in contrast to most eukaryotic lineages, is still undergoing a dramatic rate of intron gain. These novel introns carry significantly weaker splice sites that may impede their identification by the spliceosome. Novel introns are more likely to encode a premature termination codon (PTC), indicating that nonsense-mediated decay (NMD) functions as a backup for weak splicing of new introns. Our data suggest that new introns originate when genomic insertions with weak splice sites are hidden from selection by NMD. This mechanism reduces the sequence requirement imposed on novel introns and implies that the capacity of the spliceosome to recognize weak splice sites was a prerequisite for intron gain during eukaryotic evolution.

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References

Apr 20, 1979·Science·F Crick
Dec 11, 1991·Current Opinion in Genetics & Development·J D Palmer, J M Logsdon
May 1, 1991·Trends in Genetics : TIG·T Cavalier-Smith
Jul 1, 1989·Trends in Genetics : TIG·J H Rogers
May 23, 1985·Nature·T Cavalier-Smith
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·B VenkateshS Brenner
Sep 17, 2003·Genome Research·Alexei FedorovWalter Gilbert
May 5, 2004·Genome Research·Ewan BirneyRichard Durbin
Nov 25, 2004·PLoS Biology·Cydney B NielsenJames E Galagan
Apr 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Scott William Roy, Walter Gilbert
Apr 21, 2005·RNA·Xavier RocaAdrian R Krainer
Nov 18, 2005·Trends in Genetics : TIG·Daniel C JeffaresDavid Penny
May 25, 2006·Genome Biology·Haining LinC Robin Buell
May 25, 2006·Molecular Biology and Evolution·David G Knowles, Aoife McLysaght
Jul 25, 2007·Bioinformatics·Miklós CsurösIgor B Rogozin
Oct 24, 2007·Genome Biology·Jason E StajichScott W Roy
Oct 30, 2007·Molecular Biology and Evolution·Jasmin Coulombe-Huntington, Jacek Majewski
Nov 14, 2007·PLoS Genetics·Matthew W HahnSang-Gook Han
Jan 19, 2008·Nature·Olivier JaillonEric Meyer
Feb 1, 2008·Genome Biology·Thomas J SharptonJohn W Taylor
Apr 5, 2008·Science·Navtej ToorAnna Marie Pyle
Apr 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chaolin ZhangMichael Q Zhang
Apr 9, 2008·Trends in Genetics : TIG·Michael Hiller, Matthias Platzer
Apr 10, 2008·Nucleic Acids Research·Giulio PavesiGraziano Pesole
May 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rosa TarríoFrancisco Rodríguez-Trelles
Jun 6, 2008·Trends in Cell Biology·Lukas Stalder, Oliver Mühlemann
Jul 4, 2008·Trends in Genetics : TIG·Manuel IrimiaScott William Roy
Jul 29, 2008·Biochimica Et Biophysica Acta·Oliver MühlemannRodolfo Zamudio Orozco
Aug 12, 2008·Molecular Cell·Shakir SayaniGuillaume F Chanfreau
Dec 11, 2008·PLoS Biology·Francesco Catania, Michael Lynch
Dec 17, 2008·Trends in Genetics : TIG·Scott William Roy, Manuel Irimia
Feb 5, 2009·Nature Structural & Molecular Biology·Saverio Brogna, Jikai Wen
Feb 26, 2009·Cell·Markus C WahlReinhard Lührmann
Mar 31, 2009·Molecular Biology and Evolution·Jian-Qun ChenDacheng Tian
Jul 29, 2009·The Journal of Heredity·Francesco CataniaDouglas G Scofield
Aug 18, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Wenyong DingJianwu Dai
Aug 25, 2009·BMC Evolutionary Biology·Hermann RaggOlaf Krüger
Nov 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xiang Gao, Michael Lynch
Dec 8, 2009·Science·Wenli LiMichael Lynch

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Citations

Aug 16, 2014·Genome Biology and Evolution·Wenli LiMichael Lynch
Apr 18, 2012·Biology Direct·Igor B RogozinEugene V Koonin
Jun 14, 2012·International Journal of Evolutionary Biology·Dušan Kordiš, Janez Kokošar
Jun 22, 2012·PloS One·Michael MarottaHisashi Tanaka
Nov 25, 2011·Biology Direct·Dušan Kordiš
Sep 12, 2012·Biology Direct·Paul Yenerall, Leming Zhou
Sep 21, 2010·Cellular and Molecular Life Sciences : CMLS·Hermann Ragg
Sep 24, 2010·Journal of Molecular Evolution·Lei-Ying ZhangDeng-Ke Niu
Jun 6, 2013·Genome Biology and Evolution·Robin van Schendel, Marcel Tijsterman
Sep 30, 2010·EMBO Reports·Guillaume F Chanfreau
Dec 5, 2014·PLoS Genetics·Hao WangJeffrey L Bennetzen
Sep 30, 2017·Molecular Biology and Evolution·Liam Abrahams, Laurence D Hurst
Oct 25, 2014·Genome Research·Yu SunHanna Johannesson
Nov 2, 2017·Genome Research·Nicolas J TourasseDenis Dupuy
Apr 9, 2010·Nature Reviews. Genetics·Hadas KerenGil Ast
Nov 22, 2018·Frontiers in Plant Science·Claudio CapitaoKarel Riha
Jul 15, 2016·Genome Biology and Evolution·Steven Sêton Bocco, Miklós Csűrös
Nov 22, 2011·Current Biology : CB·Stefano F F TorrianiDaniel Croll
Nov 26, 2010·Trends in Genetics : TIG·Ashley FarlowChristian Schlötterer

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