Nonsense-mediated mRNA decay in yeast does not require PAB1 or a poly(A) tail.

Molecular Cell
Stacie MeauxKristian E Baker

Abstract

Eukaryotic mRNAs harboring premature translation termination codons are recognized and rapidly degraded by the nonsense-mediated mRNA decay (NMD) pathway. The mechanism for discriminating between mRNAs that terminate translation prematurely and those subject to termination at natural stop codons remains unclear. Studies in multiple organisms indicate that proximity of the termination codon to the 3' poly(A) tail and the poly(A) RNA-binding protein, PAB1, constitute the critical determinant in NMD substrate recognition. We demonstrate that mRNA in yeast lacking a poly(A) tail can be destabilized by introduction of a premature termination codon and, importantly, that this mRNA is a substrate of the NMD machinery. We further show that, in cells lacking Pab1p, mRNA substrate recognition and destabilization by NMD are intact. These results establish that neither the poly(A) tail nor PAB1 is required in yeast for discrimination of nonsense-codon-containing mRNA from normal by NMD.

References

Aug 18, 1994·Nature·D Muhlrad, R Parker
Oct 1, 1993·Genes & Development·R Pulak, P Anderson
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F HeA Jacobson
Sep 1, 1996·The Plant Journal : for Cell and Molecular Biology·A van Hoof, P J Green
Aug 29, 1997·The Journal of Biological Chemistry·A L AtkinM R Culbertson
Aug 26, 1998·Molecular and Cellular Biology·R BoeckA B Sachs
Sep 2, 1999·Human Molecular Genetics·P A Frischmeyer, H C Dietz
Sep 22, 1999·Molecular and Cellular Biology·M J Lelivelt, M R Culbertson
Feb 19, 2002·EMBO Reports·Jun WangMiles F Wilkinson
Apr 4, 2003·Current Opinion in Genetics & Development·Michael R Culbertson, Peter F Leeds
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Lynne E Maquat
May 18, 2004·Current Opinion in Cell Biology·Kristian E Baker, Roy Parker
Apr 20, 2006·Nature Structural & Molecular Biology·Marc BühlerOliver Mühlemann
May 26, 2006·Nature Reviews. Molecular Cell Biology·Nadia AmraniAllan Jacobson
Jun 20, 2006·Trends in Molecular Medicine·Holly A Kuzmiak, Lynne E Maquat
Feb 24, 2007·The EMBO Journal·Isabelle Behm-AnsmantElisa Izaurralde
Apr 18, 2007·Genes & Development·Dasa LongmanJavier F Cáceres
Jul 31, 2007·Genetics·Marenda A WilsonAmbro van Hoof

❮ Previous
Next ❯

Citations

Apr 17, 2010·Plant Cell Reports·Eduard LazarescuAndré Steinmetz
Aug 6, 2008·Nature Reviews. Genetics·Olaf Isken, Lynne E Maquat
Oct 18, 2012·Nature Reviews. Molecular Cell Biology·Stephanie Kervestin, Allan Jacobson
Feb 5, 2009·Nature Structural & Molecular Biology·Saverio Brogna, Jikai Wen
Jun 6, 2012·Nature Structural & Molecular Biology·Christopher J Shoemaker, Rachel Green
Nov 14, 2012·Briefings in Functional Genomics·Isabel M Palacios
Aug 9, 2011·Nucleic Acids Research·Stacie MeauxAmbro van Hoof
Dec 19, 2008·Journal of Virology·Jason E WeilKaren L Beemon
Nov 28, 2013·Annual Review of Genetics·Maximilian Wei-Lin Popp, Lynne E Maquat
Jul 13, 2012·Genetics·Roy Parker
Feb 19, 2014·The Journal of Cell Biology·Jens Lykke-Andersen, Eric J Bennett
Sep 10, 2011·Trends in Biochemical Sciences·Ambro van Hoof, Eric J Wagner
May 10, 2011·Current Opinion in Genetics & Development·Jungwook Hwang, Lynne E Maquat
Apr 11, 2009·Current Opinion in Cell Biology·Indrani Rebbapragada, Jens Lykke-Andersen
May 3, 2008·The EMBO Journal·Zoltán KerényiDániel Silhavy
Jun 29, 2012·Wiley Interdisciplinary Reviews. RNA·A Alejandra Klauer, Ambro van Hoof
Sep 12, 2012·Wiley Interdisciplinary Reviews. RNA·Shin-ichi Hoshino
Dec 7, 2010·Wiley Interdisciplinary Reviews. RNA·Shubhendu Ghosh, Allan Jacobson
May 3, 2014·Wiley Interdisciplinary Reviews. RNA·Callie P WigingtonAnita H Corbett
Apr 3, 2010·The EMBO Journal·Jikai Wen, Saverio Brogna
Mar 31, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jenna E Smith, Kristian E Baker
Feb 26, 2013·Biochimica Et Biophysica Acta·Christoph SchweingruberOliver Mühlemann
Sep 27, 2014·Biochimica Et Biophysica Acta·Megan PeccarelliBessie W Kebaara
May 20, 2016·Wiley Interdisciplinary Reviews. RNA·Carrie L SimmsHani S Zaher
Mar 24, 2018·Genome Research·Lorena EspinarLucas B Carey
Jul 20, 2014·Eukaryotic Cell·Megan Peccarelli, Bessie W Kebaara
Feb 21, 2014·Molecules and Cells·Maximilian Wei-Lin Popp, Lynne E Maquat
May 28, 2019·Wiley Interdisciplinary Reviews. RNA·Aparna KishorJ Robert Hogg
Jul 9, 2020·Biomolecules·Daria Lavysh, Gabriele Neu-Yilik
May 17, 2008·Biochemical Society Transactions·Jikai Wen, Saverio Brogna
Jul 8, 2020·Genetics·Joshua A ArribereHeather A Hundley
Jun 5, 2013·Journal of Cell Science·Yiwen FangShireen R Lamandé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.