Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake.

Genes & Development
Marcus J O Johansson, Allan Jacobson

Abstract

Inactivation of the yeast nonsense-mediated mRNA decay (NMD) pathway stabilizes nonsense mRNAs and promotes readthrough of premature translation termination codons. Although the latter phenotype is thought to reflect a direct role of NMD factors in translation termination, its mechanism is unknown. Here we show that the reduced termination efficiency of NMD-deficient cells is attributable to increased expression of the magnesium transporter Alr1p and the resulting effects of elevated Mg(2+) levels on termination fidelity. Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg(2+) homeostasis and, consequently, translational fidelity.

References

Jul 2, 1974·Biochemistry·A Scarpa
Jul 1, 1973·Journal of Bacteriology·G Schlanger, S M Friedman
Nov 28, 1967·Journal of Molecular Biology·M R Capecchi
Nov 1, 1994·Molecular and Cellular Biology·K A Schandel, D D Jenness
Jan 27, 1998·The Journal of Biological Chemistry·C W MacDiarmid, R C Gardner
Jun 10, 2000·Molecular and Cellular Biology·A B MaderazoA Jacobson
Jun 1, 1964·Journal of Molecular Biology·W SZER, S OCHOA
Oct 3, 2003·Chembiochem : a European Journal of Chemical Biology·Quentin Vicens, Eric Westhof
Aug 15, 2006·The FEBS Journal·Marcin WachekAnton Graschopf
Sep 9, 2006·Science·Maria SelmerV Ramakrishnan
Oct 3, 2006·Trends in Biochemical Sciences·Jan RehwinkelElisa Izaurralde
Dec 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Marcus J O JohanssonAllan Jacobson

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Citations

Sep 16, 2011·Current Genetics·Rafael Deliz-AguirreBessie W Kebaara
Oct 5, 2013·Trends in Genetics : TIG·Bijoyita Roy, Allan Jacobson
Oct 18, 2012·Nature Reviews. Molecular Cell Biology·Stephanie Kervestin, Allan Jacobson
Dec 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Magnus JohanssonMåns Ehrenberg
Apr 23, 2013·Nucleic Acids Research·Yang ZhouMarcus J O Johansson
May 2, 2012·Molecular and Cellular Biology·Igor Y MorozovMark X Caddick
Mar 24, 2012·BMC Genetics·Stephanie KvasChristopher J Brandl
Jul 9, 2011·PLoS Genetics·Isabelle ToescaGuillaume F Chanfreau
Jul 9, 2011·PloS One·Phaik Har LimColin W MacDiarmid
Jul 13, 2012·Genetics·Roy Parker
Jul 30, 2014·Nature Medicine·Andrew NicklessZhongsheng You
Dec 12, 2012·Annual Review of Medicine·Stuart W PeltzAllan Jacobson
Oct 19, 2014·Nucleic Acids Research·Inna A NikonorovaAlexey G Ryazanov
Feb 12, 2013·The Journal of Biological Chemistry·Jiajie WeiMatthew S Sachs
Oct 4, 2014·Journal of Molecular Biology·Zaineb FouratiMarc Graille
Oct 6, 2015·Annual Review of Genetics·Feng He, Allan Jacobson
Sep 27, 2014·Biochimica Et Biophysica Acta·Megan PeccarelliBessie W Kebaara
Apr 27, 2013·RNA Biology·Ana M Matia-GonzálezMiguel A Rodríguez-Gabriel
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bijoyita RoyAllan Jacobson
Aug 21, 2013·Biochimica Et Biophysica Acta·Jian PayandehEmil F Pai
Apr 28, 2016·ELife·Cosmin Saveanu, Alain Jacquier
May 18, 2016·Trends in Genetics : TIG·Saverio BrognaMarija Petric
Jul 20, 2014·Eukaryotic Cell·Megan Peccarelli, Bessie W Kebaara
May 28, 2019·Wiley Interdisciplinary Reviews. RNA·Aparna KishorJ Robert Hogg
Oct 3, 2018·RNA Biology·Fu XuMarcus J O Johansson
Mar 5, 2019·Current Drug Discovery Technologies·Saif HameedZeeshan Fatima

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