Nonsense mutations in the essential gene SUP35 of Saccharomyces cerevisiae are non-lethal

Molecular Genetics and Genomics : MGG
S ChabelskayaGalina Zhouravleva

Abstract

In the present work we have characterized for the first time non-lethal nonsense mutations in the essential gene SUP35, which codes for the translation termination factor eRF3 in Saccharomyces cerevisiae. The screen used was based on selection for simultaneous suppression of two auxotrophic nonsense mutations. Among 48 mutants obtained, sixteen were distinguished by the production of a reduced amount of eRF3, suggesting the appearance of nonsense mutations. Fifteen of the total mutants were sequenced, and the presence of nonsense mutations was confirmed for nine of them. Thus a substantial fraction of the sup35 mutations recovered are nonsense mutations located in different regions of SUP35, and such mutants are easily identified by the fact that they express reduced amounts of eRF3. Nonsense mutations in the SUP35 gene do not lead to a decrease in levels of SUP35 mRNA and do not influence the steady-state level of eRF1. The ability of these mutations to complement SUP35 gene disruption mutations in different genetic backgrounds and in the absence of any tRNA suppressor mutation was demonstrated. The missense mutations studied, unlike nonsense mutations, do not decrease steady-state amounts of eRF3.

References

Sep 1, 1992·Molecular & General Genetics : MGG·J B KopczynskiJ J Bonner
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W A WeissE C Friedberg
Dec 20, 1986·Journal of Molecular Biology·W A Weiss, E C Friedberg
Feb 1, 1985·Journal of Bacteriology·J M Song, S W Liebman
May 2, 1997·Journal of Molecular Biology·R PercudaniS Ottonello
Aug 26, 1998·Molecular and Cellular Biology·R BoeckA B Sachs
Dec 28, 1999·Annual Review of Cell and Developmental Biology·T R Serio, S L Lindquist
Feb 27, 2001·Mutation Research·Y O Chernoff
Apr 25, 2002·Nucleic Acids Research·Lance HarrellJohn F Atkins
Jul 12, 2002·Cell Motility and the Cytoskeleton·Igor A ValouevMichael D Ter-Avanesyan
Dec 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jia-Jia LiuSusan L Lindquist
Jan 7, 2003·The EMBO Journal·Lev KisselevLudmila Frolova
Feb 19, 2003·BMC Molecular Biology·Svetlana E MoskalenkoGalina A Zhouravleva
Jul 18, 2003·Biology of the Cell·Sergei Inge-VechtomovMichel Philippe
Jan 23, 2004·Nucleic Acids Research·Sanaa TorkCéline Fabret

❮ Previous
Next ❯

Citations

Jul 30, 2010·Current Genetics·Alsu F SaifitdinovaAlexey P Galkin
Aug 18, 2006·Molekuliarnaia biologiia·G A ZhuravlevaS G Inge-Vechtomov
Jun 8, 2012·Molekuliarnaia biologiia·G A Zhuravleva, V A Gryzina
Aug 21, 2007·BMC Molecular Biology·Svetlana ChabelskayaGalina Zhouravleva
Nov 15, 2011·Journal of Molecular Evolution·Peter T S van der Gulik, Wouter D Hoff
Jun 2, 2005·Reviews of Physiology, Biochemistry and Pharmacology·S RospertY Dubaquié
Dec 10, 2015·Prion·Mick F TuiteBrian S Cox
Jul 29, 2008·Molecular Microbiology·Patrick StudteRaffael Schaffrath
Mar 3, 2012·Biotechnology and Bioengineering·Byung Eun MinGyoo Yeol Jung
Apr 1, 2007·Prion·Denis KiktevGalina Zhouravleva
Oct 14, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Andrew G MatveenkoGalina A Zhouravleva
Dec 5, 2016·FEMS Yeast Research·Genrikh V SerpionovMichael D Ter-Avanesyan
May 4, 2005·Yeast
Aug 24, 2013·The Journal of Biological Chemistry·Stanislav A BondarevGalina A Zhouravleva
Mar 4, 2020·International Journal of Molecular Sciences·Nina P TrubitsinaGalina A Zhouravleva
Dec 6, 2019·Frontiers in Molecular Neuroscience·Lavrentii G DanilovStanislav A Bondarev
Jun 8, 2006·Methods : a Companion to Methods in Enzymology·Mick F Tuite, Brian S Cox

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.