PMID: 9544573Apr 17, 1998Paper

Nonspecific and immune-specific up-regulation of cytokines in rabbit dermal tuberculous (BCG) lesions

Journal of Leukocyte Biology
K SugisakiP Mounts

Abstract

To our knowledge, this is the first sequential study of cytokines in tissue sections of developing and healing tuberculous (BCG) lesions. In situ hybridization, immunohistochemical, and RT-PCR techniques were used. Cytokine mRNAs showed a biphasic pattern. The percentage of mononuclear cells (MN) containing IL-1beta, TNF-alpha, MCP-1, and IL-8 mRNAs was highest in 1- to 3-day lesions, apparently because of the nonspecific inflammatory response caused by the tubercle bacilli in the BCG vaccine. At 5 days, this percentage was significantly reduced. With IFN-gamma, the peak and trough were delayed by 2 days. By 9 days, the percentage of MN containing the mRNAs of all five cytokines had again increased and the rabbits had become tuberculin-positive. In general, MCP-1 and TNF-alpha proteins and the vascular adhesion molecules, ICAM, VCAM, and perhaps ELAM, peaked at about 3 days. Many mononuclear cells surrounding the central areas of solid and liquefied caseous necrosis contained chemokine IL-8 mRNA. IL-8 is known to attract PMN, and PMN were present nearby. In contrast, MN containing chemokine MCP-1 mRNA were present more peripherally in areas rich in macrophages and lymphocytes. The early nonspecific cytokine response seems to be...Continue Reading

Citations

Jun 1, 2018·Mediators of Inflammation·HariOm SinghR R Gangakhedkar
Apr 24, 2010·Allergology International : Official Journal of the Japanese Society of Allergology·Aya Nambu, Susumu Nakae
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C M BosioK L Elkins
Feb 27, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·C JagannathR L Hunter
Mar 8, 2012·The Journal of Infectious Diseases·Angela M MinassianHelen McShane
Sep 29, 2017·Frontiers in Immunology·Hazel M Dockrell, Steven G Smith
Jun 10, 2021·Immunity·Geetika Bajpai, Matthias Nahrendorf
Jul 6, 2021·Expert Review of Vaccines·Lazaro M Marques-NetoLuciana C C Leite

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