PMID: 3768356Oct 23, 1986Paper

Nonspecific lipid transfer protein (sterol carrier protein 2) is bound to rat liver mitochondria: its role in spontaneous intermembrane phospholipid transfer

Biochimica Et Biophysica Acta
F M MegliE Quagliariello

Abstract

In the present study we have investigated the transfer of phospholipids between vesicles and rat liver mitochondria. Transfer was measured by electron paramagnetic resonance spectroscopy using vesicles that contained spin-labeled phospholipids. A spontaneous transfer was observed which could be strongly inhibited by treating the mitochondria with the thiol reagent mersalyl. Transfer was also greatly reduced after a saline wash of the mitochondria; the transfer activity was then recovered in the wash. This activity was inhibited by tryptic digestion and mersalyl. By gel chromatography, enzyme immunoassay and immunoblotting it was demonstrated that the activity in the wash was due to the nonspecific lipid transfer protein (sterol carrier protein 2). We could estimate that up to 85% of the spontaneous phospholipid transfer between vesicles and rat liver mitochondria was mediated by this transfer protein.

References

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Citations

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D R Voelker
Nov 15, 1989·Archives of Biochemistry and Biophysics·B C McNamara, C R Jefcoate
Feb 28, 1991·Biochemical and Biophysical Research Communications·A VancuraD Haldar
Jan 1, 1988·Archives of Biochemistry and Biophysics·J Barańska, L Wojtczak
Nov 1, 1988·European Journal of Biochemistry·K TakishimaG Mamiya
Jan 20, 1995·Biochimica Et Biophysica Acta·J OstergaardJ Knudsen
Oct 18, 1996·Biochimica Et Biophysica Acta·J HuuskonenC Ehnholm
Mar 25, 1988·Biochimica Et Biophysica Acta·J BasuP Chakrabarti
May 22, 1990·Biochimica Et Biophysica Acta·L WojtczakJ Zborowski
Nov 1, 1990·Chemistry and Physics of Lipids·D G Rueckert, K Schmidt
Jan 4, 1991·Biochimica Et Biophysica Acta·J H VeerkampR G Maatman
Jun 21, 1994·Biochemistry·I HapalaF Schroeder

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