Nonstructural protein of infectious bursal disease virus inhibits apoptosis at the early stage of virus infection

Journal of Virology
Meihong Liu, Vikram N Vakharia

Abstract

Infectious bursal disease virus (IBDV), the causative agent of a highly contagious disease in chickens, carries a small nonstructural protein (NS). This protein has been implicated to play a role in the induction of apoptosis. In this study, we investigate the kinetics of viral replication during a single round of viral replication and examine the mechanism of IBDV-induced apoptosis. Our results show that it is caspase dependent and activates caspases 3 and 9. Nuclear factor kappa B (NF-kappaB) is also activated and is required for IBDV-induced apoptosis. The NF-kappaB inhibitor MG132 completely inhibited IBDV-induced DNA fragmentation, caspase 3 activation, and NF-kappaB activation. To study the function of the NS protein in this context, we generated the recombinant rGLS virus and an NS knockout mutant, rGLSNSdelta virus, using reverse genetics. Comparisons of the replication kinetics and markers for virally induced apoptosis indicated that the NS knockout mutant virus induces earlier and increased DNA fragmentation, caspase activity, and NF-kappaB activation. These results suggest that the NS protein has an antiapoptotic function at the early stage of virus infection.

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Citations

Sep 23, 2006·Virus Genes·Haisong LuAizhen Guo
Jun 10, 2011·Journal of Virology·Arun Ammayappan, Vikram N Vakharia
Dec 28, 2012·Journal of Virology·Olivier EscaffreNicolas Eterradossi
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Jul 6, 2014·In Vitro Cellular & Developmental Biology. Animal·Shahla ShahsavandiHomayoon Mahravani
Mar 29, 2011·Virus Research·Yuko Nakamura-LópezBeatriz Gómez
Jun 6, 2015·Virology·Juan Manuel CarballedaAnalía Berinstein
Aug 4, 2015·Archives of Virology·Amir KaffashiGlenn F Browning
Apr 10, 2013·Developmental and Comparative Immunology·Fiona IngraoThierry van den Berg
Oct 17, 2014·Journal of Virology·Jui-Shin Chang, Shau-Chi Chi
Jan 19, 2017·International Journal of Molecular Sciences·Yao Qin, Shijun J Zheng
Dec 7, 2007·Molecular & Cellular Proteomics : MCP·Xiaojuan ZhengJiyong Zhou
Mar 16, 2018·Journal of Virology·Liliana L Cubas-GaonaDolores Rodríguez
Sep 21, 2018·Frontiers in Microbiology·Yuhong PanAnchun Cheng
Jul 10, 2010·PLoS Pathogens·Pranav DanthiTerence S Dermody

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