Nonsuppressible insulin-like activity in human serum. 3. Differentiation of two distinct molecules with nonsuppressible ILA

The Journal of Clinical Investigation
A JakobE R Froesch


Total nonsuppressible insulin-like activity (ILA) of human plasma (measured by the adipose tissue assay) results from the additive effects of at least two distinct components. They differ in molecular size, solubility in acid-ethanol, and in thermostability. More than 90% of nonsuppressible ILA of human plasma is insoluble in acid-ethanol. Its molecular size of 100,000-150,000 remains unchanged by treatment with acid-ethanol, 5 M acetic acid-0.15 M NaCl, urea, and EDTA. It is inactivated by heat. Approximately 5% of total nonsuppressible serum ILA is soluble in acid-ethanol. The molecular weight is 6000-10,000 after partial purification on Sepadex G-75 (acetic acid-NaCl). This molecule is thermostabile for 3 hr at 80 degrees C. When the acid-ethanol soluble molecule with nonsuppressible ILA is chromatographed on Sephadex G-100 at neutral pH, it is eluted in a broad peak corresponding to a molecular weight of approximately 50,000-70,000. When rechromatographed on Sephadex G-75 (acetic acid-NaCl) its mol wt is irreversibly converted from 70,000 to 6000. Most of the insulin-like activity retained on Dowex-50 ("bound insulin") is eluted off Sephadex G-75 (acetic acid-NaCl) at the same column volume as the small molecular weight non...Continue Reading


Jun 1, 1975·Archives of Biochemistry and Biophysics·J ZapfE R Froesch
Jun 1, 1967·The Journal of Clinical Investigation·E A RasioG F Cahill
Aug 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·L B GueveraR E Bolinger
Oct 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·C Lopez Quijada, J L R-Candela
Sep 1, 1960·The Journal of Clinical Investigation·A E RENOLDM C SHEPS


Jun 1, 1991·Diabetes/metabolism Reviews·V Marks, J D Teale
Jul 1, 1994·Diabetes/metabolism Reviews·P M Clark, C N Hales
May 17, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·R GuidouxG Peters
Jan 1, 1983·The International Journal of Biochemistry·H J Cornell, A C Herington
May 1, 1977·Journal of Steroid Biochemistry·B I PosnerY Omori
Nov 1, 1979·Molecular and Cellular Endocrinology·R C FranklinA C Herington
Dec 30, 1969·Biochimica Et Biophysica Acta·J J van WykR P Weaver
Jan 1, 1976·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·J L Kostyo, C R Reagan
Mar 15, 1974·Biochemical and Biophysical Research Communications·K MegyesiD M Neville
Feb 14, 1980·The New England Journal of Medicine·L S Phillips, R Vassilopoulou-Sellin
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R L HintzJ J van Wyk
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T K Audhya, K D Gibson
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G Markelonis, O H Tae Hwan
Dec 1, 1975·The Journal of Clinical Investigation·P L Poffenbarger
Feb 1, 1976·The Journal of Clinical Investigation·C R KahnJ Roth
Jul 1, 1980·Clinics in Endocrinology and Metabolism·C R Kahn
Jun 1, 1975·Archives of Biochemistry and Biophysics·J ZapfE R Froesch
Jan 14, 1972·Nature·W H DaughadayJ J van Wyk
Jan 1, 1980·Journal of Supramolecular Structure·R A Bradshaw, J S Rubin
Jan 1, 1974·Annals of the New York Academy of Sciences·L J MarksR H Egdahl
Jun 1, 1974·Developmental Medicine and Child Neurology·R C Turner, N M Cohen
Feb 28, 2019·Frontiers in Endocrinology·Jeff M P HollyClaire M Perks

Related Concepts

Carbonic Acid Ions
Serum Proteins
Molecular Sieve Chromatography
Reduced Glutathione
Hydrogen-Ion Concentration

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.