Nontypeable Haemophilus influenzae adhesin protein E: characterization and biological activity

The Journal of Infectious Diseases
Elena RonanderKristian Riesbeck

Abstract

The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on pulmonary challenge than did mice immunized with a control peptide. Finally, anti-PE mouse antibodies from vaccinated mice prevented NTHi adhesion. Our data suggest that the ubiquitous adhesin PE plays an important role in the pathogenesis of NTHi infection.

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Citations

Sep 2, 2011·The Journal of Infectious Diseases·Teresia HallströmKristian Riesbeck
Dec 12, 2012·The Journal of Infectious Diseases·Farshid JalalvandKristian Riesbeck
Apr 20, 2010·Trends in Microbiology·Teresia Hallström, Kristian Riesbeck
Sep 3, 2010·Molecular Microbiology·Birendra SinghKristian Riesbeck
May 28, 2014·International Journal of Medical Microbiology : IJMM·Tamim Al JubairKristian Riesbeck
Feb 20, 2016·Expert Review of Vaccines·M Nadeem KhanMichael E Pichichero
Aug 11, 2016·FEBS Letters·Benjamin Luke DuellKristian Riesbeck
Jun 5, 2018·Expert Review of Vaccines·Farshid Jalalvand, Kristian Riesbeck
Jul 29, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Teresia HallströmKristian Riesbeck
Nov 30, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diana BarthelPeter F Zipfel
Apr 16, 2020·International Journal of Molecular Sciences·Seung-Hak ChoSung Soon Kim
Feb 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Birendra SinghKristian Riesbeck
Aug 14, 2020·International Journal of Molecular Sciences·Christian WegeleJulia Borkowski

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