Nontypeable Haemophilus influenzae induces sustained lung oxidative stress and protease expression

PloS One
Paul T KingStephen R Holdsworth

Abstract

Nontypeable Haemophilus influenzae (NTHi) is a prevalent bacterium found in a variety of chronic respiratory diseases. The role of this bacterium in the pathogenesis of lung inflammation is not well defined. In this study we examined the effect of NTHi on two important lung inflammatory processes 1), oxidative stress and 2), protease expression. Bronchoalveolar macrophages were obtained from 121 human subjects, blood neutrophils from 15 subjects, and human-lung fibroblast and epithelial cell lines from 16 subjects. Cells were stimulated with NTHi to measure the effect on reactive oxygen species (ROS) production and extracellular trap formation. We also measured the production of the oxidant, 3-nitrotyrosine (3-NT) in the lungs of mice infected with this bacterium. NTHi induced widespread production of 3-NT in mouse lungs. This bacterium induced significantly increased ROS production in human fibroblasts, epithelial cells, macrophages and neutrophils; with the highest levels in the phagocytic cells. In human macrophages NTHi caused a sustained, extracellular production of ROS that increased over time. The production of ROS was associated with the formation of macrophage extracellular trap-like structures which co-expressed the p...Continue Reading

References

Mar 28, 1998·American Journal of Respiratory and Critical Care Medicine·L V MöllerL van Alphen
Feb 17, 2001·American Journal of Respiratory and Critical Care Medicine·S D AaronR E Dales
Aug 14, 2001·American Journal of Respiratory and Critical Care Medicine·S D Shapiro
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Feb 4, 2006·Current Opinion in Pulmonary Medicine·Nader Eldika, Sanjay Sethi
Aug 16, 2006·Clinical and Experimental Immunology·B BarcelóA G N Agustí
Mar 17, 2007·American Journal of Respiratory Cell and Molecular Biology·Emma J NaylorSteven W Edwards
May 5, 2007·American Journal of Respiratory and Critical Care Medicine·Sanjay SethiTimothy F Murphy
Jan 31, 2009·American Journal of Respiratory and Critical Care Medicine·Gordon J GaschlerMartin R Stämpfli
Jun 6, 2009·The New England Journal of Medicine·Manuel G CosioAlvar Agusti
Sep 4, 2009·The New England Journal of Medicine·Joaquin Lamela, Francisco Vega
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Abdul HakkimArturo Zychlinsky
Jul 3, 2010·American Journal of Respiratory Cell and Molecular Biology·Sandra HodgePaul N Reynolds
Oct 20, 2010·Infection and Immunity·Richard A JuneauW Edward Swords
Jun 2, 2011·Nature Reviews. Drug Discovery·Grant R DrummondChristopher G Sobey
Oct 15, 2011·Nature Reviews. Immunology·Peter J Murray, Thomas A Wynn
Oct 15, 2011·PLoS Pathogens·Ama-Tawiah EssilfiePhilip M Hansbro
Nov 18, 2011·Mucosal Immunology·S KeelyP M Hansbro
Nov 19, 2011·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Michael W Konstan, Felix Ratjen
Feb 7, 2012·The Journal of Allergy and Clinical Immunology·Rekha ChaudhuriNeil C Thomson
Feb 22, 2012·Current Opinion in Pharmacology·Irfan Rahman, William MacNee
May 15, 2012·American Journal of Respiratory and Critical Care Medicine·Rosa FanerAlvar Agustí
Aug 2, 2012·The Journal of Clinical Investigation·Rubin M Tuder, Irina Petrache
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Alistair HarrisonRobert S Munson
Nov 13, 2012·The Journal of Allergy and Clinical Immunology·Paul T KingStephen R Holdsworth
Jan 29, 2013·Frontiers in Immunology·Olivia Z Cheng, Nades Palaniyar
Mar 12, 2013·The Journal of Infectious Diseases·Ka-Wing Wong, Williams R Jacobs
Jul 31, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Conroy Wong, Stuart Jones
Aug 27, 2013·Nature Reviews. Drug Discovery·Peter J Barnes
Jul 2, 2014·The European Respiratory Journal·Andreas HectorDominik Hartl

❮ Previous
Next ❯

Citations

Jun 27, 2015·Journal of Immunology Research·Paul T King, Roleen Sharma
Aug 31, 2016·Frontiers in Immunology·Bárbara Nery Porto, Renato Tetelbom Stein
May 26, 2017·Critical Reviews in Microbiology·Krishna Bajee SriramAllan W Cripps
May 13, 2017·The International Journal of Biochemistry & Cell Biology·Md Khadem AliJay C Horvat
Mar 28, 2017·Respirology : Official Journal of the Asian Pacific Society of Respirology·Janice M LeungSanjay H Chotirmall
Jan 20, 2017·Respirology : Official Journal of the Asian Pacific Society of Respirology·Sanjay H ChotirmallPhilip M Hansbro
Oct 31, 2019·Respirology : Official Journal of the Asian Pacific Society of Respirology·Shakti D ShuklaPhilip M Hansbro
Mar 14, 2020·Journal of Cellular and Molecular Medicine·Jing ZhangLixin Xie
May 11, 2018·BioMed Research International·Paul T King
Apr 25, 2017·Clinical & Translational Immunology·Shakti D ShuklaPhilip M Hansbro
Sep 17, 2020·Cells·Michal Santocki, Elzbieta Kolaczkowska
Jan 19, 2021·IScience·Ishan AgrawalSushmita Jha
Dec 18, 2020·American Journal of Respiratory Cell and Molecular Biology·Abigail M ShotlandAmy S McKee
Jun 25, 2021·ERJ Open Research·Paul T KingDavid S Armstrong

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
biopsies
flow cytometry
confocal microscopy
flow

Software Mentioned

NIKON
Image J

Related Concepts

Related Feeds

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.