Nonviral therapeutic cell vaccine mediates potent antitumor effects

Vaccine
Inés Moret-TataySalvador F Aliño

Abstract

Therapeutic vaccination of mice bearing melanoma tumors with our genetically modified tumor cells, via DOTAP/GM-CSF lipoplexes, results in >85% tumor growth inhibition. These fresh transfected cells (irradiated, frozen and thawed) are able to produce high amounts of GM-CSF transgene (>200 ng/10(6) cells/24 h). After vaccination, significant increases (>eight-fold) in specific antitumor membrane protein IgG1 and IgG2a are obtained only in groups vaccinated with GM-CSF-producing cells, where also the highest rates of tumor inhibition, and significantly delayed mice death (P<0.05), are observed. The antitumor response obtained is long-lasting in survivors (GM-CSF-group) from 6 months after the first tumor challenge, and a full 100% of mice survived to a second tumor challenge. All these results suggest that antitumor cell vaccines engineered by nonviral procedures are suitable for use as therapeutic vaccines with potential clinical applications.

References

Jan 1, 1992·Virchows Archiv. A, Pathological Anatomy and Histopathology·E HilarioS F Aliño
May 4, 1995·Biochimica Et Biophysica Acta·H FarhoodL Huang
Sep 5, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·I MoretS F Aliño
Dec 4, 2001·Journal of Immunological Methods·Patrick StordeurMichel Goldman
Mar 20, 2002·Cytokine & Growth Factor Reviews·Ivan Borrello, Drew Pardoll
May 7, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hidetsugu AsadaOsam Mazda
Aug 23, 2002·Cancer Immunology, Immunotherapy : CII·Stephen WardStephen Todryk
Jan 9, 2004·Cancer Gene Therapy·Inés Moret-TataySalvador F Aliño
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steven R LittleRobert Langer
Jul 20, 2004·Apoptosis : an International Journal on Programmed Cell Death·J C Schmollinger, G Dranoff

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.