Nonviral ultrasound-mediated gene delivery in small and large animal models.

Nature Protocols
Maxim BezDan Gazit

Abstract

Ultrasound-mediated gene delivery (sonoporation) is a minimally invasive, nonviral and clinically translatable method of gene therapy. This method offers a favorable safety profile over that of viral vectors and is less invasive as compared with other physical gene delivery approaches (e.g., electroporation). We have previously used sonoporation to overexpress transgenes in different skeletal tissues in order to induce tissue regeneration. Here, we provide a protocol that could easily be adapted to address various other targets of tissue regeneration or additional applications, such as cancer and neurodegenerative diseases. This protocol describes how to prepare, conduct and optimize ultrasound-mediated gene delivery in both a murine and a porcine animal model. The protocol includes the preparation of a microbubble-DNA mix and in vivo sonoporation under ultrasound imaging. Ultrasound-mediated gene delivery can be accomplished within 10 min. After DNA delivery, animals can be followed to monitor gene expression, protein secretion and other transgene-specific outcomes, including tissue regeneration. This procedure can be accomplished by a competent graduate student or technician with prior experience in ultrasound imaging or in p...Continue Reading

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Citations

Sep 13, 2020·Bioengineering·Richard E DeckerMarxa L Figueiredo
May 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tali IlovitshKatherine W Ferrara
Apr 20, 2021·Cellular and Molecular Bioengineering·Lingjie YangYuming Mu
Jun 7, 2021·Biomaterials·Kevin CollonJay R Lieberman
Aug 2, 2021·Advanced Drug Delivery Reviews·Nisi ZhangKatherine W Ferrara

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Methods Mentioned

BETA
transfection
X-ray
ELISA

Software Mentioned

ARRIVE

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