Noradrenergic neurotransmission in the ventral spinal cord: basic characteristics and effects of denervating lesions, as studied in the awake rat by microdialysis

Brain Research
G LeanzaS Stanzani

Abstract

Extracellular levels of noradrenaline (NA) were measured in the ventral horn of the lumbar spinal cord in awake unrestrained rats using in vivo microdialysis coupled to a highly sensitive radioenzymatic assay. In normal animals, baseline NA output averaged 13.4 +/- 2.2 fmol/30 microliters. KCl (100 mM) or desipramine (5 microM) added to the perfusion fluid increased NA levels 11.2-fold and 2.2-fold, respectively, whereas neuronal impulse blockade by tetrodotoxin (1 microM) added in the presence of desipramine stimulation produced a 88% reduction of extracellular NA levels. Noradrenergic denervation of the spinal cord by either electrolytic destruction of the noradrenaline-containing axon terminals or intraventricular 6-hydroxydopamine produced, 3-4 weeks later, dramatic 84 and 91% reductions in baseline NA release associated to a marked loss of immunoreactive noradrenergic fibers throughout the spinal cord or caudal to the site of electrolytic damage and almost completely abolished responses to pharmacological manipulations. The results support the view that spinal extracellular NA levels are neuronally derived, also suggesting that noradrenergic neurotransmission in the ventral spinal cord largely (by at least 85%) depends on ...Continue Reading

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Citations

Aug 14, 1999·Brain Research. Brain Research Protocols·H GustafssonE Brodin
Dec 2, 2005·Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders : Official Publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases·L SalauzeM Geffard
Apr 4, 2000·Physiological Reviews·J C ReklingJ L Feldman

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