NorB, an efflux pump in Staphylococcus aureus strain MW2, contributes to bacterial fitness in abscesses.

Journal of Bacteriology
Yanpeng DingDavid C Hooper

Abstract

While remaining a major problem in hospitals, Staphylococcus aureus is now spreading in communities. Strain MW2 (USA400 lineage) and other community methicillin-resistant S. aureus strains most commonly cause skin infections with abscess formation. Multidrug resistance (MDR) efflux pumps contribute to antimicrobial resistance but may also contribute to bacterial survival by removal of environmental toxins. In S. aureus, NorA, NorB, NorC, and Tet38 are chromosomally encoded efflux pumps whose overexpression can confer MDR to quinolones and other compounds (Nor pumps) or tetracyclines alone (Tet38), but the natural substrates of these pumps are not known. To determine the role of these efflux pumps in a natural environment in the absence of antibiotics, we used strain MW2 in a mouse subcutaneous abscess model and compared pump gene expression as determined by reverse transcription-PCR in the abscesses and in vitro. norB and tet38 were selectively upregulated in vivo more than 171- and 24-fold, respectively, whereas norA and norC were downregulated. These changes were associated with an increase in expression of mgrA, which encodes a transcriptional regulator known to affect pump gene expression. In competition experiments using e...Continue Reading

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Citations

Aug 10, 2013·The Journal of Antimicrobial Chemotherapy·Yee Gyung KwakDavid C Hooper
Sep 14, 2011·Antimicrobial Agents and Chemotherapy·Brian J MorrowA Simon Lynch
Sep 22, 2010·Antimicrobial Agents and Chemotherapy·Jody L FloydManuel F Varela
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Jun 17, 2009·Antimicrobial Agents and Chemotherapy·Hong Bin KimDavid C Hooper
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