Norcantharidin blocks Wnt/β-catenin signaling via promoter demethylation of WIF-1 in glioma

Oncology Reports
Dajiang XieShuxu Yang

Abstract

Glioma is one of the most common primary intracranial tumors, and the prognosis is poor even though much treatment management is employed. Wnt/β-catenin signaling has been reported to be associated with glioma. Norcantharidin (NCTD) is the demethylated analog of cantharidin isolated from blister beetles, and it is reported to possess anticancer activity but less nephrotoxicity than cantharidin. Accordingly, we aimed to investigate NCTD as an anti-neoplastic drug that inhibits the Wnt/β‑catenin pathway via promoter demethylation of Wnt inhibitory factor-1 (WIF-1) in glioma growth in vitro. In the present study, we report that NCTD inhibited cell proliferation, induced apoptosis and cell cycle arrest, and suppressed cell migration and invasion in vitro. Moreover, we observed that the expression levels of WIF-1 mRNA and protein in the NCTD-treated cells were increased significantly compared with these levels in the negative control (NC) cells. Promoter demethylation was observed in the NCTD‑treated cells. In contrast, aberrant methylation was observed in the NC cells. Additionally, more investigation revealed that NCTD suppressed activity of Wnt/β-catenin signaling and transcription of β-catenin/TCF-4. Furthermore, the expression ...Continue Reading

References

Mar 26, 2010·Journal of Experimental & Clinical Cancer Research : CR·Zhuanyi YangYanjin Wang
Sep 14, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·K A ChuangY C Kuo
Nov 30, 2011·Cancer Biology & Therapy·Shujing LiuXiaowei Xu
Feb 18, 2012·Molecular Biology of the Cell·Aaron PitreOmar Skalli
May 23, 2012·Connective Tissue Research·Dongwu LiuXiuzhen Zhang
Jul 11, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·You-Ming JiangJia-Xu Chen
Aug 21, 2012·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Jun WuYanjin Wang
Aug 25, 2012·TheScientificWorldJournal·Rebecca SchüleChristel Herold-Mende
Jan 19, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sun A KimSeong Who Kim
Apr 25, 2013·International Journal of Molecular Medicine·Sansan ChenJinlong Li
Sep 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Chen-Hsi HsiehYu-Jen Chen
Nov 2, 2013·Journal of Neuro-oncology·An-An YinXiang Zhang
Nov 19, 2013·Expert Review of Anticancer Therapy·Amade BregyRicardo J Komotar
Dec 7, 2013·Neuro-oncology·Sabit DelicMarkus J Riemenschneider
May 3, 2014·Biochemical and Biophysical Research Communications·Xiangrong ChenJunyan Chen
May 14, 2014·Seminars in Cancer Biology·Cara JamiesonBeric R Henderson
Jul 16, 2014·World Journal of Surgical Oncology·Jie ZhengXin-Ting Wei
Aug 31, 2014·Pharmacology & Therapeutics·Phuong N LeAntonio Jimeno
Oct 7, 2014·International Journal of Molecular Sciences·Andrew J SchneiderRichard E Peterson

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