Nordihydroguaiaretic acid blocks protein transport in the secretory pathway causing redistribution of Golgi proteins into the endoplasmic reticulum.

The Journal of Biological Chemistry
T FujiwaraY Ikehara

Abstract

We have investigated the effect of nordihydroguaiaretic acid (NDGA), an inhibitor of lipoxygenase, on the intracellular protein transport and the structure of the Golgi complex. Pulse-chase experiments and immunoelectron microscopy showed that NDGA strongly inhibits the transport of newly synthesized secretory proteins to the Golgi complex resulting in their accumulation in the endoplasmic reticulum (ER). Despite their retention in the ER, oligosaccharides of secretory and ER-resident proteins were processed to endoglycosidase H-resistant forms, raising the possibility that oligosaccharide-processing enzymes are redistributed from the Golgi to the ER. Morphological observations further revealed that alpha-mannosidase II (a cis/medial-Golgi marker), but not TGN38 (a trans-Golgi network marker), rapidly redistributes to the ER in the presence of NDGA, resulting in the disappearance of the characteristic Golgi structure. Upon removal of the drug, the Golgi complex was reassembled into the normal structure as judged by perinuclear staining of alpha-mannosidase II and by restoration of the secretion. These effects of NDGA are quite similar to those of brefeldin A. However, unlike brefeldin A, NDGA did not cause a dissociation of bet...Continue Reading

References

Nov 1, 1977·The Journal of Experimental Medicine·A M Tartakoff, P Vassalli
Jun 15, 1992·Biochemical and Biophysical Research Communications·T FujiwaraY Ikehara
Jan 30, 1992·Nature·J E Rothman, L Orci
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Oct 1, 1970·Biochemical Pharmacology·R S PardiniD C Fletcher
Jun 10, 1981·European Journal of Pharmacology·C L ArmourD M Temple
Aug 1, 1995·Current Opinion in Cell Biology·N R Salama, R W Schekman
Apr 26, 1995·Biochemical and Biophysical Research Communications·T KinouchiK Suzuki
Dec 1, 1994·The Journal of Cell Biology·O MartinezB Goud
Nov 3, 1994·Nature·J E Rothman
Nov 16, 1994·Biochemical Pharmacology·M PavaniJ Ferreira
Dec 30, 1994·Cell·H R Pelham
Dec 3, 1993·Cell·J OstermannJ E Rothman
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Apr 12, 1996·Science·J E Rothman, F T Wieland
May 1, 1996·Journal of Biochemistry·M TagayaS Mizushima
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·O MartinezB Goud
Jul 14, 1997·The Journal of Cell Biology·S I Bannykh, W E Balch
Oct 6, 1997·The Journal of Biological Chemistry·T YamaguchiM Tagaya

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Oct 25, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jin-Shiung Cheng, Chung-Ren Jan
Jan 10, 2002·Biochemical and Biophysical Research Communications·Anja ten BrinkeArie B Vaandrager
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Aug 22, 2008·Journal of Virology·Benno WölkCharles M Rice
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Nov 13, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·I GátiN Dizdar
Aug 23, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gulam H Syed, Aleem Siddiqui
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Oct 3, 2002·Journal of Biochemistry and Molecular Biology·Sun-Mee KimJin-Woo Park
Apr 20, 2014·Biochimica Et Biophysica Acta·Hercules Antônio da Silva-SouzaPedro Muanis Persechini
Mar 30, 2001·Fungal Genetics and Biology : FG & B·V KhalajG D Robson
Jul 16, 2014·Antiviral Research·Rubén Soto-AcostaRosa M Del Angel
Jun 3, 2017·Drug Metabolism Reviews·Sofía López-GilVarenka J Barbero-Becerra
Oct 24, 2003·The Journal of Biological Chemistry·Siro SimizuHiroyuki Osada
Sep 25, 2004·Journal of Cellular Physiology·Hidenori ItoKanefusa Kato
Aug 1, 2014·American Journal of Physiology. Cell Physiology·Hercules A da Silva-SouzaEliana Scemes
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Oct 3, 2003·The Journal of Biological Chemistry·Eiji OhtaYukio Ikehara
Apr 16, 2002·European Journal of Biochemistry·Vincent Th G SchoonderwoertGerard J M Martens
Nov 5, 2002·Journal of Cell Science·Masami NagahamaMitsuo Tagaya
Jan 27, 1999·Biochemical and Biophysical Research Communications·T FujiwaraY Ikehara
Aug 20, 2005·Experimental and Molecular Pathology·Amy K McNally, James M Anderson
Oct 28, 2004·European Journal of Cell Biology·Nils BäckBetty A Eipper

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