Nordihydroguaiaretic acid (NDGA) blocks the differentiation of C2C12 myoblast cells

Journal of Cellular Physiology
Hidenori ItoKanefusa Kato

Abstract

Addition of nordihydroguaiaretic acid (NDGA) to the differentiation medium of C2C12 mouse myoblast cells caused severe inhibition of the formation of myotubes and suppressed differentiation-dependent elevation in the levels of the creatine kinase M isozyme (CKM). Under these conditions, NDGA did not cause significant increase of damaged cells, as detected by annexin-V-FITC assay, or induction of heat shock proteins, known to be a response against extracellular stress. The results suggest that NDGA itself is not toxic but can effectively blocks the differentiation-dependent increase of CKM during C2C12 differentiation. The levels of muscle specific bHLH proteins MyoD, Myf5, and myogenin were also decreased by addition of NDGA, indicating a block of the initial step of the myogenesis through downregulation of muscle specific genes. NDGA is known to be a lipoxygenase inhibitor but other examples, like MK-886 and CDC, did not exert the same effects on differentiation of muscle cells, indicating that mechanisms of NDGA action are independent of its influence on lipoxygenase.

References

Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J BlakeN J Holbrook
Apr 1, 1991·The Journal of Clinical Investigation·S J Tapscott, H Weintraub
Apr 1, 1991·Journal of Medicinal Chemistry·H ChoT Ishihara
Jan 1, 1986·Annual Review of Biochemistry·P NeedlemanJ B Lefkowith
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·D M GuidotJ E Repine
Mar 7, 1997·The Journal of Biological Chemistry·S A CoolicanJ R Florini
Feb 5, 2000·Developmental Biology·S M Davidson, M Morange
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Marpadga A ReddyRama Natarajan
May 8, 2003·The Journal of Biological Chemistry·Melissa E HatleyCatherine C Hedrick

❮ Previous
Next ❯

Citations

Dec 29, 2016·Antioxidants & Redox Signaling·Emmeran Le MoalRémi Mounier
Nov 13, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·I GátiN Dizdar
Aug 30, 2011·Apoptosis : an International Journal on Programmed Cell Death·Ujwal Mukund MahajanKulbhushan Tikoo
Oct 24, 2019·Current Cancer Drug Targets·Geraldine Sandana Mala JohnSuresh Kumar Rayala

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.