Norepinephrine modulates osteoarthritic chondrocyte metabolism and inflammatory responses

Osteoarthritis and Cartilage
J LorenzSusanne G Grässel

Abstract

Norepinephrine (NE) was measured in synovial fluid of trauma patients and sympathetic nerve fibers were detected in healthy and osteoarthritic (OA) joint tissues indicating that cartilage pathophysiology might be influenced by sympathetic neurotransmitters. The aim of this study was to elucidate the mostly unknown role of NE in OA chondrocyte metabolism and inflammatory responses. Articular cartilage was received after total knee replacement surgery from OA patients. Expression of adrenergic receptors (AR) and tyrosine hydroxylase (TH) was tested with end point polymerase chain reaction (PCR) and immunohistochemistry. 3-dimensional (3D) cell cultures were employed to analyze effects of NE on chondrocyte cell metabolism and the expression of interleukins (ILs), matrix metalloproteases (MMPs), tissue inhibitor of metalloproteases (TIMPs), glycosaminoglycan (GAG) and collagen II under non- and inflammatory conditions. Chondrocyte monolayer cultures were used to specify AR subtypes, to analyze cell cycle distribution and to determine catecholamines in cell culture supernatants. AR subtypes and TH were detected in chondrocytes, whereas NE was not released in measurable amounts. 10(-6) M NE reversed IL-1β induced changes in IL-8, MMP...Continue Reading

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Citations

Apr 30, 2017·International Journal of Molecular Sciences·Susanne Grässel, Dominique Muschter
May 3, 2018·Physiological Reviews·Florent Elefteriou
Oct 7, 2017·Disease Models & Mechanisms·Sandra FoertschAnita Ignatius
Feb 27, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaomin KangShufang Wu
Jan 18, 2019·International Journal of Molecular Sciences·Katarzyna D SluzalskaJuergen Steinmeyer
Mar 22, 2020·International Journal of Molecular Sciences·Johannes KupkaZsuzsa Jenei-Lanzl
Jun 30, 2019·International Journal of Molecular Sciences·Karima El BagdadiZsuzsa Jenei-Lanzl
Mar 14, 2019·International Journal of Molecular Sciences·Saskia SpeichertZsuzsa Jenei-Lanzl
Sep 5, 2019·The Veterinary Clinics of North America. Small Animal Practice·Alexander Valverde, Alicia M Skelding
Feb 12, 2021·Cellular Signalling·Rebecca SohnZsuzsa Jenei-Lanzl
Oct 8, 2017·Journal of Biomechanics·Carley N McCutchenRonald K June
Aug 25, 2021·BMC Musculoskeletal Disorders·Hyun Sook HwangHyun Ah Kim

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