Normal colonic epithelium adheres to carcinoembryonic antigen and type IV collagen

Gastroenterology
S IshiiJ M Jessup

Abstract

Human colorectal carcinoma cells bind to collagen and laminin in the basement membrane as well as to carcinoembryonic antigen (CEA) on neighboring cells. The purpose of this study was to determine whether normal colonic epithelial cells bind to CEA, collagen, or laminin. Intact colonic crypts were isolated from normal mucosa in 13 specimens resected for colorectal carcinoma or colonic diverticulitis. Colonocytes were released from the crypts by treatment with collagenase and deoxyribonuclease and tested for adhesion to CEA, type IV collagen, and laminin in a solid-phase adhesion assay. Twelve percent to 25% of colonocytes in all specimens bound to CEA. Colonocytes from seven specimens also bound to type IV collagen, but none of the colonocyte preparations bound significantly to laminin. Monoclonal antibodies to CEA and to the hyaluronate receptor CD44 and enzymatic removal of membrane CEA blocked the adhesion of colonocytes to CEA. First, colonocytes use the same epitopes on CEA and CD44 as colorectal carcinoma cells to adhere to solid-phase CEA. Second, colonocytes bind to solid-phase CEA through CEA-to-CEA homophilic binding. Third, CEA and type IV collagen, but not laminin, are adhesion ligands for human colonocytes.

Citations

Jan 1, 1997·In Vitro Cellular & Developmental Biology. Animal·C Ilantzis, C P Stanners
May 20, 1998·International Journal of Cancer. Journal International Du Cancer·R S BresalierC K Yunker
Aug 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·H OgawaS Matsuno
Dec 1, 1996·The Australian and New Zealand Journal of Surgery·M V Agrez
Jan 23, 1999·Japanese Journal of Cancer Research : Gann·T OgoshiS Matsuno
Dec 1, 1995·Cancer Metastasis Reviews·S JothyO W Blaschuk
Dec 1, 1996·Scandinavian Journal of Gastroenterology·M RieglerE Wenzl
May 20, 1998·Clinical and Experimental Pharmacology & Physiology·M D Basson
Aug 1, 1996·Digestive Diseases and Sciences·E C Ebert
Sep 8, 1998·Seminars in Surgical Oncology·J M Jessup, M Loda
Jan 1, 1997·Critical Reviews in Oncology/hematology·Y MatsushitaE Sato

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.