PMID: 3771639Oct 1, 1986Paper

Normal human epidermis contains an interferon-like protein

The Journal of Cell Biology
M YaarB A Gilchrest

Abstract

Interferons have been postulated to participate in growth regulation of normal body tissues and are known to inhibit growth of human epidermal keratinocytes in vitro. Polyclonal antibodies to recombinant human interferon-alpha, purified by passage over an affinity column (Sepharose coupled to the recombinant interferon), used in the indirect immunofluorescent method specifically stained the proliferative (basal) compartment of human epidermis in histological cross-sections of normal skin and in cultured keratinocyte colonies. Extracts prepared from healthy nonvirally infected keratinocyte cultures contained interferon activity as determined by viral plaque inhibition assay. Using the Western blotting technique column-purified antibodies and antisera to recombinant human interferon-alpha recognized a band of approximately 40 kD when reacted with both extracted keratinocyte proteins and recombinant human interferon-alpha standards, that gave in addition a band of approximately 20 kD. The above findings suggest that interferon or a closely related protein is present in the proliferative compartment of normal epidermis in the absence of viral infection and therefore may serve as a physiological modulator of epidermal growth.

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Citations

Oct 1, 1990·Journal of Cellular Physiology·M YaarB A Gilchrest
Jan 1, 1989·Archives of Dermatological Research·J K LivdenR Matre
Jan 1, 1989·Experimental Gerontology·M PeacockeB A Gilchrest
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J W FountainJ Lahti
Jun 14, 2000·Cancer Biotherapy & Radiopharmaceuticals·D R BielenbergC D Bucana
Feb 6, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·H FujisawaD N Sauder
Sep 1, 1992·The British Journal of Dermatology·B A Gilchrest, M Yaar
Oct 1, 1990·The Journal of Dermatologic Surgery and Oncology·M Yaar, B A Gilchrest
Oct 1, 1992·Experimental Dermatology·B M Stanulis-PraegerB A Gilchrest
Mar 1, 1990·Journal of Clinical Pathology·C J StewartA K Foulis
Jan 24, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Peter J BuontempoBahige M Baroudy
Jan 1, 1988·Annals of the New York Academy of Sciences·R TigelaarP Bergstresser
Jan 1, 1988·Annals of the New York Academy of Sciences·M YaarB A Gilchrest
May 3, 2014·Seminars in Oncology·Hyeonjoo CheonGeorge R Stark
Jul 4, 2019·Nature Communications·Ekaterini PlatanitisThomas Decker
Aug 22, 2001·The Journal of Biological Chemistry·D W LaFleurP A Moore
Jun 5, 2017·Scientific Reports·Diwakar SanthakumarMuhammad Munir
Dec 12, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Justin Taft, Dusan Bogunovic
Aug 1, 1993·The Journal of Investigative Dermatology·E K ParkinsonI B Pragnell

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