PMID: 9159405Jan 1, 1997Paper

Normal human fibroblasts immortalized by introduction of human papillomavirus type 16 (HPV-16) E6-E7 genes

Microbiology and Immunology
T ShigaB Simizu

Abstract

This report demonstrates that normal human fibroblasts can be immortalized by the introduction of HPV-16 E6-E7 genes. We designed zinc-inducible expression plasmids with HPV-16 E6, E7 or both. Each plasmid was introduced into normal human fibroblasts (TIG-3 cells) using lipofection methods. Only transfectants with the HPV-16 E6-E7 zinc-inducible expression plasmid, which were cultured in medium supplemented with 100 microM ZnSO4, overcame crisis and could be cultured over 200 population doubling levels (PDLs). These cell lines showed the reactivation of telomerase after crisis, and morphological alterations were also observed.

References

Jan 1, 1982·Journal of Gerontology·M MatsuoK Hosoda
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M CounterC B Harley

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Citations

Jun 11, 1999·Journal of Cellular Physiology·M C MontaltoF A Ray
Oct 3, 2008·In Vitro Cellular & Developmental Biology. Animal·Kerry Claydon, Leigh Owens
Dec 13, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Sudip SenNeeta Singh
Jan 23, 2009·Journal of Cellular Biochemistry·Hyun Min JungJin Kyeoung Kim
Dec 8, 2011·Microbiology and Immunology·Hussein H AlyTsukasa Seya
Oct 31, 2006·The International Journal of Biochemistry & Cell Biology·Yi-Wei WangWinston C Y Yu
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Akito YamamotoTakeki Tsutsui
Jul 20, 2006·Molecular Biology Reports·Xinwen WangYan Jin

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