Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells

Skeletal Muscle
Dimuthu K Wasgewatte WijesingheCharles N Pagel

Abstract

Osteopontin is secreted by skeletal muscle myoblasts and macrophages, and its expression is upregulated in muscle following injury. Osteopontin is present in many different structural forms, which vary in their expression patterns and effects on cells. Using a whole muscle autograft model of muscle injury in mice, we have previously shown that inflammation and regeneration of muscle following injury are delayed by the absence of osteopontin. The current study was undertaken to determine whether muscle or non-muscle cells provide the source of osteopontin required for its role in muscle regeneration. The extensor digitorum longus muscle of wild-type and osteopontin-null mice was removed and returned to its bed in the same animal (autograft) or placed in the corresponding location in an animal of the opposite genotype (allograft). Grafts were harvested at various times after surgery and analysed by histology, flow cytometry and quantitative polymerase chain reaction. Data were analysed using one- or two-way ANOVA or Kruskal-Wallis test. Immunohistochemistry confirmed that osteopontin was expressed by macrophages in osteopontin-null muscle allografts in wild-type hosts and by myoblasts in wild-type allografts in osteopontin-null h...Continue Reading

References

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Citations

Oct 11, 2019·Biomarkers in Medicine·Fabrizio MontecuccoFederico Carbone
Jan 4, 2020·Development·Alan Y Wong, Jessica L Whited
Mar 3, 2020·Frontiers in Physiology·Emily E HowardNancy R Rodriguez
Jun 20, 2020·Current Atherosclerosis Reports·Hao DengXijuan Jiang
Jul 15, 2021·APL Bioengineering·Cooper BlakeRichard J Williams

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Methods Mentioned

BETA
flow cytometry
FACS
ELISA
FCS
of
PCR
Fluorescence

Software Mentioned

FACS Diva
gene
SPOT
FlowJo
Primer
Adobe Photoshop
GraphPad Prism

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