Normal oxygen atmosphere is essential for the solitary long-term culture of early preantral mouse follicles

Molecular Reproduction and Development
J SmitzA C Van Steirteghem

Abstract

This study compares the effects of reduced (5%) or normal (5% CO2 in air; 20% O2) oxygen tension on the in vitro maturation of early preantral ovarian follicles isolated from 14-day-old (C57BI/6J x CBAca) F1 mice. Intact follicles (100-130 microns) are singly cultured in 20 microliters droplets alpha-MEM enriched with FCS and rFSH under mineral oil at 37 degrees C and 100% humidity. In this culture system the follicles are allowed to attach to the bottom of the petri dishes. Follicle in vitro growth, hormone secretory capacity, and in vitro ovulation were studied under the two oxygen tensions. Spontaneous oocyte release from the follicle during a 16-day culture period was observed significantly more under 5% oxygen. Antral-like cavity formation was not observed under 5% O2. The follicles in the 5% O2 cultures reaching day 16 were stripped of their granulosa cell layers, and 83% of the retrieved oocytes had already undergone spontaneous germinal-vesicle breakdown (GVBD). Under 20% O2, the GV stage was maintained until day 16 in 77% of the oocytes. Under 5% O2, intact follicle survival up to day 12 was significantly reduced as compared to the 5% CO2 in air conditioning. The hCG stimulus on day 12 induced mucification in a signifi...Continue Reading

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Citations

Aug 21, 2003·Archives of Gynecology and Obstetrics·Jochen ReinsbergHans van der Ven
Jan 1, 1999·Maturitas·J Smitz, R Cortvrindt
Nov 6, 2004·Journal of Assisted Reproduction and Genetics·Dong-Hoon KimHoon-Taek Lee
Nov 25, 2003·Tissue Engineering·Stephanie A PangasTeresa K Woodruff
Dec 21, 2006·The Journal of Reproduction and Development·Shu HashimotoYoshiharu Morimoto
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Jul 7, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Yuji Hirao
Mar 16, 2011·Annals of the New York Academy of Sciences·Joshua Johnson, Pasquale Patrizio
Mar 20, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Jaesook Roh, Woojin Pi
Feb 20, 1999·Biology of Reproduction·S CecconiM Mattioli
Jun 29, 2012·The Journal of Reproduction and Development·Yuji Hirao
Jun 4, 2019·Reproduction in Domestic Animals = Zuchthygiene·Leda Maria Costa PereiraNucharin Songsasen
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Mar 4, 2011·Reproduction, Fertility, and Development·Joanne E FortuneWanzirai Muruvi
Apr 13, 2015·Reproduction, Fertility, and Development·J M ConnollyA C Hynes

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