PMID: 2112584Jun 1, 1990Paper

Normal recombination substrate VH to DJH rearrangements in pre-B cell lines from scid mice

The Journal of Experimental Medicine
P FerrierF W Alt

Abstract

To further analyze the VDJ recombination defect in lymphoid pre-B cells from mice with severe combined immune deficiency (scid mice), we have assayed the ability of Abelson murine leukemia virus (A-MuLV) transformed pre-B cells from scid mice to rearrange a recombination substrate in which inverted VH to DJH joins activate a selectable (gpt) gene. In unselected populations, substrate rearrangements occurred frequently, but were aberrant and probably analogous to the aberrant rearrangements observed at endogenous scid Ig gene loci. In contrast, populations of scid pre-B lines selected for gpt activity within the substrate contained mostly "normal" VH to DJH joins within the introduced substrate. These findings demonstrate that scid pre-B cells can make normal joins at low efficiency and are discussed with respect to the potential mechanism of the scid defect and the occurrence of Igs in leaky scid mice.

References

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Citations

Aug 1, 1992·Immunology Today·F W AltJ Chen
Apr 1, 1991·Current Opinion in Immunology·J M McCune
Mar 2, 2002·Immunology·Kirk S HinkleyJames E Riggs
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E A HendricksonD T Weaver
Oct 1, 1991·The Journal of Experimental Medicine·L J KienkerP W Tucker
Dec 1, 1993·The Journal of Experimental Medicine·D B KotloffN R Ruetsch
Jun 24, 1998·The Journal of Experimental Medicine·J P ManisF W Alt
Mar 5, 2003·The Journal of Experimental Medicine·Sean RooneyJoAnn Sekiguchi
Dec 1, 1991·Immunological Reviews·J ReimannM H Claesson
Jul 10, 2004·Immunological Reviews·Maya Goldmit, Yehudit Bergman
Jul 19, 2006·Veterinary Pathology·R SeymourH Hogenesch
Nov 1, 1991·European Journal of Immunology·C A ReynaudJ C Weill
Oct 1, 1990·Molecular and Cellular Biology·E A HendricksonD T Weaver
Oct 1, 1996·Molecular and Cellular Biology·J S DanskaC J Guidos
Jun 1, 1991·Molecular and Cellular Biology·E A HendricksonD T Weaver
Feb 1, 1993·Molecular and Cellular Biology·J T Meier, S M Lewis

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