Normal replication of vesicular stomatitis virus without C proteins

Virology
E KretzschmarJ K Rose

Abstract

The expression of two small basic proteins (C and C') encoded by a second open reading frame of the New Jersey serotype of vesicular stomatitis virus (VSV) P gene was reported previously (Spiropoulou and Nichol, J. Virol., 67, 3103-3110, 1993). Here we found that the Indiana serotype virus also expressed C and C' proteins from this reading frame. We eliminated C and C' expression by making a single base change that introduced a stop codon in the C and C' coding sequence, but left the P-protein sequence unchanged. This mutated P gene supported normal replication and packaging of VSV minigenomes encoding G and M proteins. The mutated P gene was also recombined into an infectious clone of VSV that was used to recover virus. The mutant virus no longer expressed the C and C' proteins but showed growth kinetics identical to wild-type virus. The amounts of viral mRNAs and proteins synthesized were indistinguishable in mutant and wild-type virus infected cells as were the yields and composition of mutant and wild-type virus particles. The kinetics of host protein-synthesis shut-off were also identical for both viruses. Although the C and C' proteins were dispensable for VSV growth in tissue culture, they are known to be conserved in al...Continue Reading

Citations

Oct 16, 2012·Virus Genes·Martin PfefferManfred Weidmann
Jan 1, 2012·Uirusu·Akira Nishizono, Kentaro Yamada
Jul 16, 2009·Archives of Virology·Hong-Lian ChenYu-Lin Jiang
Aug 11, 2011·Archives of Virology·Steven J PauszekLuis L Rodriguez
Jun 11, 1998·Trends in Biotechnology·A García-Sastre
Dec 19, 2003·Protein Expression and Purification·Mark A MillerMichael A Whitt
Sep 22, 2011·Virus Research·Peter J WalkerKim R Blasdell
Nov 30, 2004·Virus Research·Himangi R JayakarMichael A Whitt
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Aug 2, 2014·The Journal of General Virology·Laure BellecLaurent Bigarré
Apr 1, 1997·Reviews in Medical Virology·F Radecke, M A Billeter
Feb 10, 2000·Molecular Biology and Evolution·I K JordanM A McClure
Sep 3, 2020·Viruses·Christiane RiedelKarl-Klaus Conzelmann

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