Normal specification of the extraembryonic lineage after somatic nuclear transfer

FEBS Letters
Satoshi KishigamiTeruhiko Wakayama

Abstract

To examine the establishment and maintenance of trophectoderm (TE) lineage in somatic cloned blastocysts, the expression of Cdx2, a key molecule for specification of TE fate, was immunohistochemically examined simultaneously with Oct4 expression. Cloned mouse embryos were made by nuclear transfer using cumulus cells, tail-tip fibroblasts, and embryonic stem cells. After 96 h of culture, the rates of Oct4-expressing blastocysts were as low as 50% and 60% for cumulus and fibroblast clones, respectively. However, regardless of Oct4 expression, the majority of those cloned blastocysts (> 90%) normally expressed Cdx2. Thus, even though somatic cloned embryos have reduced potential to produce the inner cell mass lineage, the TE lineage can be established and maintained.

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Citations

Jan 8, 2010·Biomedical Microdevices·Eva M CampoJose Antonio Plaza
Apr 15, 2010·Development, Growth & Differentiation·Mayumi OdaSatoshi Tanaka
Mar 6, 2010·The Journal of Reproduction and Development·Nguyen Van ThuanTeruhiko Wakayama
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mayumi OdaKunio Shiota
Jul 30, 2014·Cellular Reprogramming·Anna MallolElena Ibáñez
Jun 1, 2010·Biochemical and Biophysical Research Communications·Yingying WangQi Zhou
Oct 22, 2008·Molecular Reproduction and Development·Miyuri KawasumiKazuya Matsumoto
Apr 27, 2010·Developmental Biology·S T BalbachM Boiani
Feb 6, 2015·Biology of Reproduction·Eiji MizutaniAtsuo Ogura
Nov 6, 2007·Biology of Reproduction·Yuko JinchoTomohiro Kono
Aug 2, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Alexander Meissner, Rudolf Jaenisch
Nov 11, 2019·The Journal of Biological Chemistry·Nanami KohriManabu Kawahara

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