Normalisation genes for expression analyses in the brown alga model Ectocarpus siliculosus.

BMC Molecular Biology
Aude Le BailBénédicte Charrier

Abstract

Brown algae are plant multi-cellular organisms occupying most of the world coasts and are essential actors in the constitution of ecological niches at the shoreline. Ectocarpus siliculosus is an emerging model for brown algal research. Its genome has been sequenced, and several tools are being developed to perform analyses at different levels of cell organization, including transcriptomic expression analyses. Several topics, including physiological responses to osmotic stress and to exposure to contaminants and solvents are being studied in order to better understand the adaptive capacity of brown algae to pollution and environmental changes. A series of genes that can be used to normalise expression analyses is required for these studies. We monitored the expression of 13 genes under 21 different culture conditions. These included genes encoding proteins and factors involved in protein translation (ribosomal protein 26S, EF1alpha, IF2A, IF4E) and protein degradation (ubiquitin, ubiquitin conjugating enzyme) or folding (cyclophilin), and proteins involved in both the structure of the cytoskeleton (tubulin alpha, actin, actin-related proteins) and its trafficking function (dynein), as well as a protein implicated in carbon metab...Continue Reading

References

Feb 20, 1995·Molecular & General Genetics : MGG·K E AptA R Grossman
Aug 4, 2001·Environment International·M GraymoreG Allinson
Jul 18, 2002·Trends in Plant Science·Elizabeth Blée
Jun 14, 2003·Science·S L Baldauf
Jun 23, 2004·Journal of Experimental Botany·Claire GachonBénédicte Charrier
Jul 28, 2004·Chemosphere·S Giacomazzi, N Cochet
Aug 20, 2004·BMC Plant Biology·Amy M BrunnerSteven H Strauss
Jun 16, 2005·Journal of Molecular Endocrinology·S A BustinM W Pfaffl
Sep 29, 2005·Journal of Experimental Botany·Nathalie NicotDanièle Evers
May 13, 2006·Biochemical and Biophysical Research Communications·Mukesh JainJitendra P Khurana
Jun 15, 2007·BMC Developmental Biology·Karen GoossensLuc J Peelman
Jan 10, 2008·The New Phytologist·Bénédicte CharrierJ Mark Cock
Jan 30, 2008·BMC Molecular Biology·Manuel Pombo-SuarezAntonio Gonzalez

❮ Previous
Next ❯

Citations

Apr 25, 2012·Extremophiles : Life Under Extreme Conditions·Chenlin LiuBailin Cong
Dec 8, 2011·Biofouling·Emmanuelle EvaristeJames A Callow
Dec 6, 2011·Omics : a Journal of Integrative Biology·Thierry TononAnne Siegel
Dec 11, 2013·Journal of Experimental Botany·Agnès GroisillierThierry Tonon
Oct 1, 2013·Nucleic Acids Research·Bernard BilloudBénédicte Charrier
Mar 5, 2010·Plant Physiology·Aude Le BailBénédicte Charrier
Aug 12, 2009·BMC Molecular Biology·Neil I Bower, Ian A Johnston
Jan 14, 2011·BMC Molecular Biology·Simon M DittamiThierry Tonon
Feb 6, 2014·PloS One·Nathalie KowalczykJonas Collén
Nov 5, 2010·Journal of Microbiological Methods·François ThomasGurvan Michel
Mar 5, 2016·Frontiers in Microbiology·Javier E TapiaJuan A Correa
Mar 8, 2012·The Plant Journal : for Cell and Molecular Biology·Simon M DittamiThierry Tonon
Nov 10, 2011·Plant Signaling & Behavior·Susana M CoelhoJ Mark Cock
Feb 26, 2015·Frontiers in Plant Science·Denis Saint-MarcouxBénédicte Charrier
Jun 24, 2017·The New Phytologist·Marina Klemenčič, Christiane Funk
Dec 29, 2017·Parasites & Vectors·Sook-Luan NgKien-Hui Chua
Sep 24, 2013·Molecular Biology Reports·Arash Kianianmomeni, Armin Hallmann
May 28, 2019·Genes·Frédéric SanchezNigel Grimsley
Mar 7, 2021·International Journal of Molecular Sciences·Pramod RathorBalakrishnan Prithiviraj
Dec 5, 2017·Current Biology : CB·Andrea Del CortonaOlivier De Clerck

❮ Previous
Next ❯

Methods Mentioned

BETA
chemical treatments
chemical treatment

Software Mentioned

Primer
Normfinder
geNorm
BestKeeper

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.