Normalization of voltage-sensitive dye signal with functional activity measures.

PloS One
Kentaroh TakagakiFrank W Ohl

Abstract

In general, signal amplitude in optical imaging is normalized using the well-established DeltaF/F method, where functional activity is divided by the total fluorescent light flux. This measure is used both directly, as a measure of population activity, and indirectly, to quantify spatial and spatiotemporal activity patterns. Despite its ubiquitous use, the stability and accuracy of this measure has not been validated for voltage-sensitive dye imaging of mammalian neocortex in vivo. In this report, we find that this normalization can introduce dynamic biases. In particular, the DeltaF/F is influenced by dye staining quality, and the ratio is also unstable over the course of experiments. As methods to record and analyze optical imaging signals become more precise, such biases can have an increasingly pernicious impact on the accuracy of findings, especially in the comparison of cytoarchitechtonic areas, in area-of-activation measurements, and in plasticity or developmental experiments. These dynamic biases of the DeltaF/F method may, to an extent, be mitigated by a novel method of normalization, DeltaF/DeltaF(epileptiform). This normalization uses as a reference the measured activity of epileptiform spikes elicited by global disi...Continue Reading

References

Jan 1, 1978·Annual Review of Neuroscience·L B CohenA Grinvald
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·K R DelaneyD Kleinfeld
Dec 22, 2000·Progress in Neurobiology·Y Momose-SatoK Kamino
Oct 10, 2003·Journal of Neurophysiology·Hong-Tao MaJian-Young Wu
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carl C H PetersenBert Sakmann
Jan 22, 2004·Nature Reviews. Neuroscience·Mircea Steriade
Mar 9, 2004·Journal of Neuroscience Methods·A L ObaidB M Salzberg
Oct 22, 2004·Nature Reviews. Neuroscience·Amiram Grinvald, Rina Hildesheim
Nov 5, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaoying HuangJian-Young Wu
Jan 27, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hartwig SporsRainer W Friedrich
Feb 24, 2006·Nature·Tatyana O SharpeeKenneth D Miller
Mar 31, 2006·Journal of Neurophysiology·Eugene F Civillico, Diego Contreras
Aug 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Per E RolandBashir Ahmed
May 12, 2007·Journal of Neurophysiology·Michael T LippertJian-Young Wu
Jul 6, 2007·Neuron·Weifeng XuJian-young Wu
Nov 13, 2007·Journal of Electrocardiology·Guy Salama, Bum-Rak Choi
Dec 7, 2007·Neuron·Isabelle FerezouCarl C H Petersen
Jan 8, 2008·Neuroscience Letters·Kentaroh TakagakiMichael Thomas Lippert
Jul 23, 2008·Bulletin of Experimental Biology and Medicine·S Yu KriganovskayaL E Molokanova

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single-chip

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Matlab
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