PMID: 9420027Jan 7, 1998Paper

Nosocomial spread of cephem-resistant Escherichia coli strains carrying multiple Toho-1-like beta-lactamase genes

Antimicrobial Agents and Chemotherapy
Tetsuya YagiY Arakawa

Abstract

Escherichia coli HKY56, which demonstrated resistance to various beta-lactams except carbapenems, was isolated from the throat swab of an inpatient in 1994. Conjugal transfer of cephem resistance from HKY56 to E. coli CSH2 was not successful. Three cefotaxime-resistant E. coli clones harboring plasmid pMRE001, pMRE002, or pMRE003, each of which carried a 3.4-, 5.8-, or 6.2-kb EcoRI fragment insert, respectively, were obtained from HKY56. Although restriction analysis suggested their different origins, these clones showed similar profiles of resistance to various beta-lactams. The sequence of 10 amino acid residues at the N terminus of beta-lactamase purified from E. coli HB101(pMRE001) was identical to that of Toho-1. This Toho-1-like beta-lactamase-1 (TLB-1) was able to hydrolyze cefoperazone and cefotaxime efficiently, but it failed to hydrolyze cephamycins. A Toho-1-specific DNA probe was hybridized with three distinct EcoRI fragments derived from the chromosomal DNA of strain HKY56, and these fragments corresponded to DNA inserts carried by pMRE001, pMRE002, and pMRE003, respectively. PCR and Southern hybridization analysis suggested that all six cephem-resistant E. coli strains, strains HKY273, HKY285, HKY288, HKY305, HKY3...Continue Reading

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Citations

Jul 14, 2001·Antimicrobial Agents and Chemotherapy·R BonnetJ Sirot
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Aroonwadee ChanawongPeter Michael Hawkey
Oct 15, 2005·Clinical Microbiology Reviews·David L Paterson, Robert A Bonomo
Jan 27, 2006·Antimicrobial Agents and Chemotherapy·Naohiro ShibataYoshichika Arakawa
Jan 25, 2013·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Masaki Yoshida
Feb 19, 2020·Microbiology and Immunology·Yoshichika Arakawa
Dec 10, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D L Paterson, V L Yu
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·R Bonnet
Apr 24, 2004·Canadian Journal of Microbiology·Jan Walther-Rasmussen, Niels Høiby
Dec 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jesús Rodríguez-BañoAlvaro Pascual
Nov 14, 2008·The Journal of Antimicrobial Chemotherapy·Satowa SuzukiYoshichika Arakawa
Aug 25, 2011·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·Saori YasunagaNaohiro Shibata
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·George A Jacoby
Jun 20, 2000·Antimicrobial Agents and Chemotherapy·R BonnetJ Sirot
Jan 26, 2002·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Y. HirakataShigeru Kohno
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·Marcela RadiceGabriel Gutkind
Mar 8, 2005·Journal of Clinical Microbiology·Yoshikazu IshiiKeizo Yamaguchi
Dec 10, 2003·Journal of Clinical Microbiology·Noriyuki NaganoYoshichika Arakawa

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