Not just fat: investigating the proteome of cetacean blubber tissue

Conservation Physiology
Joanna L KershawAilsa J Hall

Abstract

Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This study presents a first step towards the systematic analysis of the protein content of cetacean adipose tissue, the blubber, in order to investigate the kinds of proteins present and their relative abundance. Full depth blubber subsamples were collected from dead-stranded harbour porpoises (Phocoena phocoena) (n = 21). Three total protein extraction methods were trialled, and the highest total protein yields with the lowest extraction variability were achieved using a RIPA cell lysis and extraction buffer based protocol. Extracted proteins were separated using 1D Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), and identified using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI-MS/MS). A range of proteins were identified (n = 295) and classed into eight functional groups, the most abundant of which were involved in cell func...Continue Reading

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Citations

Oct 27, 2018·Biology Open·Jane I KhudyakovCory D Champagne
Jul 2, 2020·Ecology and Evolution·Juliana Castrillon, Susan Bengtson Nash
Dec 2, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jared DeyarminJane Khudyakov
May 1, 2021·Life·Jayan D M Senevirathna, Shuichi Asakawa

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