Notch-1 Signaling Activation and Progesterone Receptor Expression in Ectopic Lesions of Women With Endometriosis

Journal of the Endocrine Society
Dustin M BrownRosalia C M Simmen

Abstract

Progesterone (P) resistance is a hallmark of endometriosis, but the underlying mechanism(s) for loss of P sensitivity leading to lesion establishment remains poorly understood. To evaluate the association between Notch-1 signaling activation and P resistance in the progression of endometriosis. Case control study; archived formalin-fixed, paraffin-embedded tissues. University hospitals (United States, Taiwan). Women with endometriosis; human endometrial stromal cell line (HESC). Eutopic endometria (EU) and ectopic lesions (ECs) were collected from surgically diagnosed patients. Archived tissue sections of EU and ECs were identified. HESCs were treated with N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) and valproic acid (VPA) to, respectively, suppress and induce Notch-1 activation. Tissues were analyzed for Notch Intra-Cellular Domain 1 (NICD1) and progesterone receptor (PGR) protein expression by immunohistochemistry and for transcript levels of NICD1 target genes HES1, PGR, and PGR-B by quantitative reverse transcription polymerase chain reaction. DAPT- or VPA-treated HESCs with and without P cotreatment were evaluated for cell numbers and for PGR, HES1, and PGR target gene DKK1 transcript level...Continue Reading

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Citations

Feb 13, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·Na LiWenqian Xiong
Aug 20, 2019·Acta Obstetricia Et Gynecologica Scandinavica·Laura Ramirez WilliamsMartin Götte
Feb 13, 2020·Reproductive Sciences·John Mark P PabonaRosalia Cm Simmen
Jun 23, 2020·Chinese Medical Journal·Zhi-Yue GuJin-Hua Leng
Aug 8, 2019·International Journal of Molecular Sciences·Ryan M MarquardtJae-Wook Jeong
Sep 5, 2021·Trends in Endocrinology and Metabolism : TEM·Genna E MoldovanAsgerally T Fazleabas
Oct 30, 2021·Frontiers in Physiology·Melissa E Heard-LipsmeyerRosalia C M Simmen

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Methods Mentioned

BETA
PCR

Software Mentioned

Primer Express
SPSS
SigmaStat

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