Notch inhibits osteoblast differentiation and causes osteopenia.

Endocrinology
Stefano ZanottiErnesto Canalis

Abstract

Notch receptors are determinants of cell fate decisions. To define the role of Notch in the adult skeleton, we created transgenic mice overexpressing the Notch intracellular domain (NICD) under the control of the type I collagen promoter. First-generation transgenics were small and osteopenic. Bone histomorphometry revealed that NICD caused a decrease in bone volume, secondary to a reduction in trabecular number; osteoblast and osteoclast number were decreased. Low fertility of founder mice and lethality of young pups did not allow the complete establishment of transgenic lines. To characterize the effect of Notch overexpression in vitro, NICD was induced in osteoblasts and stromal cells from Rosa(notch) mice, in which a STOP cassette flanked by lox(P) sites is upstream of NICD, by transduction with an adenoviral vector expressing Cre recombinase (Cre) under the control of the cytomegalovirus (CMV) promoter (Ad-CMV-Cre). NICD impaired osteoblastogenesis and inhibited Wnt/beta-catenin signaling. To determine the effects of notch1 deletion in vivo, mice in which notch1 was flanked by lox(P) sequences (notch1(loxP/loxP)) were mated with mice expressing Cre recombinase under the control of the osteocalcin promoter. Conditional null...Continue Reading

References

Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J LianG Stein
Dec 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A M ParfittR R Recker
Feb 1, 1993·Experimental Cell Research·M Lardelli, U Lendahl
Feb 28, 1998·The Biochemical Journal·T C Dale
Sep 8, 1998·Current Opinion in Genetics & Development·B M Gumbiner
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Wodarz, R Nusse
Apr 16, 1999·The EMBO Journal·T OhtsukaR Kageyama
Apr 30, 1999·Science·S Artavanis-TsakonasR J Lake
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·W SongB A Yankner
Jul 20, 1999·Molecular and Cellular Biology·C S Wesley
Jun 23, 2000·The Journal of Clinical Investigation·P Bianco, P Gehron Robey
Nov 10, 2000·Developmental Biology·E NollK S Kosik
Mar 10, 2001·Genes & Development·P A Sharp
Mar 27, 2001·The Journal of Cell Biology·B De Strooper, W Annaert
Aug 4, 2001·Molecular and Cellular Biology·T IsoY Hamamori
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I KalajzicD Rowe
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ken-Ichi TezukaNobumichi Hozumi
Feb 12, 2002·International Journal of Molecular Medicine·Michael SchnabelJürgen Schlegel
Apr 12, 2002·Journal of Cellular Biochemistry·Rosa M R PereiraErnesto Canalis
Apr 25, 2002·Nucleic Acids Research·Irina NazarenkoAyoub Rashtchian
Jul 19, 2002·Current Biology : CB·Daniel R FoltzJeffrey S Nye
Sep 10, 2003·Endocrinology·Maria SciaudoneErnesto Canalis
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·L Charles MurtaughDouglas A Melton
Aug 10, 2004·Journal of Mammary Gland Biology and Neoplasia·Robert Callahan, Sean E Egan
Oct 12, 2004·Gene·Jennifer J WestendorfTania M Schroeder
Nov 13, 2004·Endocrinology·Elisabetta GazzerroErnesto Canalis
Jan 25, 2005·Nature Immunology·Andrew W DuncanTannishtha Reya
Jun 17, 2005·Nature·Silvia FreSpyros Artavanis-Tsakonas

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Citations

Oct 18, 2011·Calcified Tissue International·Stefano Zanotti, Ernesto Canalis
Jul 8, 2010·Journal of Bone and Mineral Metabolism·Hironori HojoUng-il Chung
Jun 4, 2011·Journal of Bone and Mineral Metabolism·Gloria H Y LiQing-Yang Huang
Mar 23, 2013·Current Osteoporosis Reports·Jenna Regan, Fanxin Long
Dec 20, 2011·Endocrine·G MazziottiA Giustina
Dec 23, 2011·Nature Reviews. Molecular Cell Biology·Fanxin Long
Jul 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Timothy J Mead, Katherine E Yutzey
Dec 7, 2010·The Journal of Biological Chemistry·Stefano ZanottiErnesto Canalis
Nov 19, 2010·The Journal of Biological Chemistry·Stefano ZanottiErnesto Canalis
Jan 18, 2012·The Journal of Experimental Medicine·Baohong ZhaoLionel B Ivashkiv
Apr 14, 2012·Human Molecular Genetics·Yangjin BaeBrendan H Lee
Apr 28, 2009·Current Opinion in Nephrology and Hypertension·Koichi Matsuo
Dec 10, 2009·Molecular and Cellular Biology·Stefano Zanotti, Ernesto Canalis
Jan 1, 2013·Endocrinology·Ernesto CanalisStefano Zanotti
Apr 5, 2013·European Journal of Endocrinology·Stefano Zanotti, Ernesto Canalis
Aug 22, 2013·Calcified Tissue International·Shan ChenYangjin Bae
Mar 29, 2014·Der Orthopäde·R BeckmannT Pufe
Jun 4, 2014·The Journal of Clinical Investigation·Hengwei ZhangLianping Xing
Aug 10, 2010·Annual Review of Genomics and Human Genetics·Dustin BaldridgeBrendan Lee
Aug 22, 2009·Journal of Molecular and Cellular Cardiology·Vishal Nigam, Deepak Srivastava
Mar 8, 2016·The Journal of Clinical Investigation·Cuicui WangMatthew J Hilton
Sep 20, 2011·Journal of Cellular Biochemistry·Stefano Zanotti, Ernesto Canalis
Aug 6, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michael I DishowitzKurt D Hankenson
Apr 5, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Laura Rose, Hasan Uludağ
May 26, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jianning TaoBrendan Lee
Nov 6, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Brya G MatthewsIvo Kalajzic
Apr 16, 2010·Annals of the New York Academy of Sciences·Jianning TaoBrendan Lee
Jun 19, 2013·Journal of Biomedical Materials Research. Part a·Michael I DishowitzKurt D Hankenson
Dec 31, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Tong-Han ZhangGuang-Sen Zheng
Apr 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Peng LiuMone Zaidi
Sep 10, 2014·Cancer Cell·Jianning TaoBrendan H Lee
Jan 18, 2015·Molecular and Cellular Endocrinology·Ye TianYufeng Dong
Dec 11, 2014·Orphanet Journal of Rare Diseases·Ernesto Canalis, Stefano Zanotti

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