Notch signaling regulates osteosarcoma proliferation and migration through Erk phosphorylation

Tissue & Cell
Jie QinDong Wang

Abstract

We used a murine spontaneous osteosarcoma cell line with high metastatic potential, the K7M2 cell line to study the role of Notch signaling in the biological manifestations of osteosarcoma, to understand its underlying mechanism in the regulation of cell proliferation and migration, and to improve patient prognosis in cases of osteosarcoma through the discovery of novel therapeutic targets, First, Notch expression in K7M2 was determined by immunostaining, and the γ-secretase inhibitor N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) was used to inhibit proteolytic cleavage of the Notch intracellular domain (NICD), resulting in the inhibition of Notch activation. By using the Sulforhodamine B assay, colony-forming units assay, Brdu and Ki67 staining, and flow cytometry assays of apoptosis and cell cycle stage, DAPT was found to inhibit K7M2 proliferation in a dose-dependent manner. By using wound healing and transwell migration assays, DAPT was found to inhibit K7M2 migration in a dose-dependent manner as well. By using a combination of micro-Raman spectroscopy and K-means clustering analysis, we found that DAPT inhibit a variety of important cell metabolism-related components in most K7M2 cell struct...Continue Reading

Citations

Jan 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min-Hong HsiehAnd Ko-Hsiu Lu
Oct 13, 2020·Tissue Engineering. Part B, Reviews·Kathleen Zohorsky, Kibret Mequanint
Mar 31, 2021·Journal of Orthopaedic Surgery and Research·Jianping ZhangYongqing Xu
Jul 14, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vandit ShahJigna Shah

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