Notch1 controls cell chemoresistance in small cell lung carcinoma cells

Thoracic Cancer
Wael Abdo HassanTakaaki Ito

Abstract

Small cell lung carcinoma (SCLC) is characterized by a high rate of relapse and failure of chemotherapy because of the emergence of drug resistant cells. Notch signaling controls carcinogenesis in several human malignancies and could be involved in the resistance of cells to several chemotherapeutic agents. Herein, we analyzed the role of Notch1 signaling in the resistance of human SCLC cells to doxorubicin. Small interfering ribonucleic acid technology was used to knock down (KD) Notch1 in H69AR and SBC-3 SCLC cells. We detected the effect of inhibiting Notch1 on the expression of drug resistant related molecules: multidrug resistance-associated protein (MRP-1) and anti-apoptotic factor B-cell lymphoma-2, as well as to cell adhesion molecule E-cadherin, which contributes to the adhesion of SCLC cells to the extracellular matrix and confers chemoresistance in a process known as cell adhesion-mediated drug resistance (CAM-DR). We also observed the effect of KD Notch1 on cell survival under high concentrations of doxorubicin treated media. H69AR and SBC-3 cells expressed Notch1 protein and grew as adherent aggregates, which confer resistance to high concentrations of doxorubicin. On inhibiting Notch1, we observed activation of th...Continue Reading

References

Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y I ChoiR H Seong
Oct 23, 2003·Nature Reviews. Cancer·Freddy Radtke, Kenneth Raj
Feb 21, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lucio Miele
May 3, 2006·Cancer Research·Sathish Kumar MungamuriKumaravel Somasundaram
May 14, 2008·Reviews on Recent Clinical Trials·Barbara Fischer, Alexandre Arcaro
Sep 29, 2011·The Journal of Experimental Medicine·Camille LobryIannis Aifantis
Dec 1, 2012·Archives of Pathology & Laboratory Medicine·Paul A Bunn
Jan 4, 2013·Asian Cardiovascular & Thoracic Annals·Till PloenesBernward Passlick
Jun 4, 2014·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hassan WaelTakaaki Ito
Dec 3, 2014·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Wael Abdo HassanTakaaki Ito

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Citations

Nov 17, 2016·Oxidative Medicine and Cellular Longevity·Angelo SparaneoLucia Anna Muscarella
Apr 11, 2019·Cellular Oncology (Dordrecht)·Alessandro LeonettiMarcello Tiseo
Sep 12, 2020·Molecular Pharmacology·Lobna Faiz GharaibehWalhan Alshaer

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