Notch1 inhibition reduces low shear stress-induced plaque formation

The International Journal of Biochemistry & Cell Biology
Wei-dong QinMingxiang Zhang

Abstract

Low shear stress (LSS) contributes to the pathogenesis of inflammatory diseases, such as atherosclerosis. Notch1 is a type I transmembrane receptor that critically determines the growth, differentiation, and survival of various cell types, but its role and mechanism in LSS-induced inflammatory response remains undetermined. Apolipoprotein E-deficient (ApoE(-/-)) mice were fed with high fat diet and administered intraperitoneally with DAPT (a γ-secretase inhibitor). Perivascular shear stress modifiers were placed around the right carotid arteries to induce LSS. The left carotid arteries with undisturbed shear stress (USS) were used as the control. LSS increased Delta-like 1 (DLL-1) protein expression and the expression of Notch1 and NICD, while DAPT administration reduced NICD expression. Compared with the LSS group, DAPT reduced LSS-induced plaque formation and intercellular adhesion molecule 1 (ICAM-1) expression. Human umbilical vein endothelial cells (HUVECs) were exposure to undisturbed shear stress (USS, 1Pa) or LSS (0.4Pa). Notch1 was inhibited by siRNA or DAPT. RT-PCR and western blotting analysis showed that LSS upregulated the expression of Notch1 in a time-dependent manner. Caveolin-1 (CAV1) inhibition by siRNA could ...Continue Reading

References

Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·F LogeatA Israël
Apr 30, 1999·Science·S Artavanis-TsakonasR J Lake
Dec 4, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S S NijjarA J Strain
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Dec 20, 2002·Nature·Peter Libby
May 6, 2003·Annual Review of Neuroscience·Dennis Selkoe, Raphael Kopan
Jun 21, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Victor RizzoPeter F Davies
Apr 16, 2004·Immunity·Todd N EagarJeffrey A Bluestone
Apr 28, 2004·Current Opinion in Genetics & Development·Andrew P Weng, Jon C Aster
Feb 26, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Menno P J de WintherMarten H Hofker
Apr 22, 2005·The New England Journal of Medicine·Göran K Hansson
Aug 22, 2006·Nature Reviews. Molecular Cell Biology·Sarah J Bray
Dec 19, 2007·European Journal of Immunology·Tanapat PalagaBarbara A Osborne
May 23, 2009·Developmental Cell·Mark E Fortini
Aug 18, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Douglas NamHanjoong Jo
Apr 20, 2010·Expert Review of Cardiovascular Therapy·Saurabh S DhawanHabib Samady
Aug 27, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hasina Hamilton OuttzJan Kitajewski
Jul 9, 2011·Stroke; a Journal of Cerebral Circulation·Zelan WeiSic L Chan
Oct 23, 2012·Biochimica Et Biophysica Acta·Wei-dong QinMingxiang Zhang
Oct 23, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Chetan P HansVidu Garg
May 2, 2014·Molecules and Cells·Kyung-Sun HeoJun-ichi Abe
Apr 2, 2015·PloS One·Espen D JahnsenElizabeth A V Jones

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Citations

Aug 28, 2016·Developmental Biology·Bryce LaFoyaAllan R Albig
Aug 27, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Toshiaki NakanoMasanori Aikawa
Mar 23, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Celine Souilhol, Paul C Evans
Jan 27, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Rachel BoardmanMaria J C Machado
Aug 31, 2016·Molecular Medicine Reports·Zhiyou CaiGuanghui Chen
Aug 2, 2019·Nature Reviews. Cardiology·Celine SouilholPaul C Evans
Nov 29, 2020·Free Radical Biology & Medicine·Jinna ChenDangheng Wei

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