Notch2 and Proteomic Signatures in Mouse Neointimal Lesion Formation.

Arteriosclerosis, Thrombosis, and Vascular Biology
Sarah M PetersonLucy Liaw

Abstract

Vascular remodeling is associated with complex molecular changes, including increased Notch2, which promotes quiescence in human smooth muscle cells. We used unbiased protein profiling to understand molecular signatures related to neointimal lesion formation in the presence or absence of Notch2 and to test the hypothesis that loss of Notch2 would increase neointimal lesion formation because of a hyperproliferative injury response. Murine carotid arteries isolated at 6 or 14 days after ligation injury were analyzed by mass spectrometry using a data-independent acquisition strategy in comparison to uninjured or sham injured arteries. We used a tamoxifen-inducible, cell-specific Cre recombinase strain to delete the Notch2 gene in smooth muscle cells. Vessel morphometric analysis and immunohistochemical staining were used to characterize lesion formation, assess vascular smooth muscle cell proliferation, and validate proteomic findings. Loss of Notch2 in smooth muscle cells leads to protein profile changes in the vessel wall during remodeling but does not alter overall lesion morphology or cell proliferation. Loss of smooth muscle Notch2 also decreases the expression of enhancer of rudimentary homolog, plectin, and annexin A2 in va...Continue Reading

References

May 4, 2000·The American Journal of Pathology·K J HarmonV Lindner
Apr 15, 2003·The Journal of Biological Chemistry·Alexey FomenkovEdward Ratovitski
Dec 26, 2003·The American Journal of Pathology·Daniel L Myers, Lucy Liaw
Jan 21, 2004·Cardiovascular Research·Joost P G SluijterDominique P V de Kleijn
Mar 9, 2004·Molecular Biology of the Cell·Jagath R JunutulaRichard H Scheller
Nov 22, 2005·British Journal of Pharmacology·Barbara RinaldiAmelia Filippelli
Jan 7, 2006·Genesis : the Journal of Genetics and Development·Brent McCrightThomas Gridley
Mar 15, 2006·Cardiovascular Drugs and Therapy·Arno RuusaleppGuro Valen
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Chiara CantariniJack R Wands
Aug 1, 2007·The Journal of Biological Chemistry·Philip S HodkinsonTariq Sethi
Apr 24, 2008·Lab Animal·J Camille EfflerTrenton R Schoeb
Jun 10, 2008·Analytical Chemistry·Gordana IvosevRon Bonner
Dec 18, 2009·British Journal of Pharmacology·Robert A McDonaldRoger M Wadsworth
Apr 16, 2010·Nature·Yan WuChristian W Siebel
Nov 12, 2010·Development·Xuesong FengThomas Gridley
Jan 5, 2011·Circulation Research·Aikaterini TsaousiSarah J George
Feb 19, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Vincenza CaoloMark J Post
Feb 19, 2011·Nature·Scott E WilliamsElaine Fuchs
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Joseph F Arboleda-VelasquezSpyros Artavanis-Tsakonas
Jun 18, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Xiaojing WuIssei Komuro
Jul 2, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Jason L JohnsonAndrew C Newby
Aug 2, 2011·Nature Medicine·Utpal B PajvaniDomenico Accili
Aug 2, 2011·Seminars in Thrombosis and Hemostasis·Klaus T Preissner, Ute Reuning
Aug 25, 2011·Pathology, Research and Practice·Fusun GundoganSuzanne M de la Monte
May 24, 2012·Circulation Research·Holly C WilliamsKathy Griendling

❮ Previous
Next ❯

Citations

Dec 24, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Hong S LuAlan Daugherty
Dec 29, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Karla X Vazquez-PradaHang T Ta

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR
transgenic
SMA
proteomic profiling

Software Mentioned

PeakView
MarkerView
PANTHER GeneOntology
PANTHER GO - Slim
ProteinPilot
SWATH
ImageJ
PANTHER
REACTOME
GraphPad Prism

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.