Note: A simple image processing based fiducial auto-alignment method for sample registration

The Review of Scientific Instruments
Wesley D RobertsonR J Dwayne Miller

Abstract

A simple method for the location and auto-alignment of sample fiducials for sample registration using widely available MATLAB/LabVIEW software is demonstrated. The method is robust, easily implemented, and applicable to a wide variety of experiment types for improved reproducibility and increased setup speed. The software uses image processing to locate and measure the diameter and center point of circular fiducials for distance self-calibration and iterative alignment and can be used with most imaging systems. The method is demonstrated to be fast and reliable in locating and aligning sample fiducials, provided here by a nanofabricated array, with accuracy within the optical resolution of the imaging system. The software was further demonstrated to register, load, and sample the dynamically wetted array.

References

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May 1, 2010·Physical Chemistry Chemical Physics : PCCP·Kresimir Franjic, Dwayne Miller
Dec 23, 2010·Analytical Chemistry·Arash Zarrine-AfsarR J Dwayne Miller
Jun 7, 2011·Nature Methods·Derron BishopThomas Misgeld
May 1, 1987·IEEE Transactions on Pattern Analysis and Machine Intelligence·J Illingworth, J Kittler
Jan 31, 2012·Analytical Chemistry·Kamila ChughtaiKristine Glunde
Feb 22, 2012·Acta Crystallographica. Section D, Biological Crystallography·Arash Zarrine-AfsarR J Dwayne Miller
Jan 5, 2014·Lab on a Chip·Mohammad Ali KhorshidiHelene Andersson Svahn
Mar 7, 2014·The Review of Scientific Instruments·Timothy R McJunkinJill R Scott
Apr 9, 2014·Advanced Materials·Ermanno MieleFrancesco De Angelis

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