PMID: 8939035Nov 1, 1996Paper

Note: comparison of media for the isolation of lactose-positive Salmonella

The Journal of Applied Bacteriology
J RuizM A Usera

Abstract

We studied the capacity of 10 selective media (Rambach agar, RB; salmonella-shigella agar, SS; SM-ID medium, SM; Hektoen enteric agar, HE; modified semisolid Rappaport-Vassiliadis agar, MSRV; bismuth sulphite agar, BS; MacConkey agar, MC; brilliant green agar, BG; novobiocin-brilliant green-glucose agar, NBG; and novobiocin-brilliant green-glycerol-lactose agar, NBGL), and the C8-esterase test (MUCAP test, Biolife, Italy) to detect the growth of 14 strains of lactose-positive Salmonella (12 Salm. virchow and two Salm. montevideo) and 16 Salm. arizonae. Suspensions of pure strain were plated on the aforementioned media and on Mueller-Hinton, used as a control, with inocula of 3 x 10(2) cfu ml-1. The performance of BS was excellent, determining the 30 strains as typical Salmonella colonies (H2S+). On NBG, 27 strains were detected. On MSRV, only some strains grew and only one produced swarming. On the other media, the two Salm. montevideo and the 12 Salm. virchow strains produced coliform colonies. Some of these latter were inhibited on BG and NBGL. The 16 Salm. arizonae strains produced typical colonies on all the media, except on RB, SM and MSRV. On NBGL, two strains did not produce H2S. The C8-esterase test was only successful ...Continue Reading

References

Oct 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S T AspinallD N Hutchinson
Feb 1, 1992·Journal of Clinical Microbiology·J RuizJ Gomez
Aug 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J RuizJ Oliva
Oct 1, 1991·Journal of Clinical Microbiology·A M Freydiere, Y Gille
Sep 1, 1986·Applied and Environmental Microbiology·J A DevenishM H Brodsky
Apr 1, 1983·Journal of Clinical Microbiology·S D Dube
May 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J RuizJ Gómez

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Citations

Jan 29, 1999·International Journal of Food Microbiology·E de Boer

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