Notoginsenoside R1 inhibits vascular smooth muscle cell proliferation, migration and neointimal hyperplasia through PI3K/Akt signaling

Scientific Reports
Haihong FangJun Yu

Abstract

Restenosis caused by neointimal hyperplasia significantly decreases long-term efficacy of percutaneous transluminal angioplasty (PTA), stenting, and by-pass surgery for managing coronary and peripheral arterial diseases. A major cause of pathological neointima formation is abnormal vascular smooth muscle cell (VSMC) proliferation and migration. Notoginsenoside R1 (NGR1) is a novel saponin that is derived from Panax notoginseng and has reported cardioprotective, neuroprotective and anti-inflammatory effects. However, its role in modulating VSMC neointima formation remains unexplored. Herein, we report that NGR1 inhibits serum-induced VSMC proliferation and migration by regulating VSMC actin cytoskeleton dynamics. Using a mouse femoral artery endothelium denudation model, we further demonstrate that systemic administration of NGR1 had a potent therapeutic effect in mice, significantly reducing neointimal hyperplasia following acute vessel injury. Mechanistically, we show that NGR1's mode of action is through inhibiting the activation of phosphatidylinositol 3-kinase (PI3K)/Akt signaling. Taken together, this study identified NGR1 as a potential therapeutic agent for combating restenosis after PTA in cardiovascular diseases.

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Citations

Sep 19, 2019·Nutrition and Cancer·Huijuan TangMonica Mattioli-Belmonte
Dec 5, 2018·Journal of Cellular Physiology·Yang HeRenping Huang
Oct 23, 2019·Natural Product Research·Ji-Wu HuangDong-Ming Zhang
Oct 13, 2020·Circulation Research·Redouane AherrahrouMete Civelek
Jun 21, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Bang-Yue LuoJing-Wei Shao
Nov 1, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hanbing LiuXiaohui Fan

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Methods Mentioned

BETA
percutaneous transluminal angioplasty
electrophoresis

Software Mentioned

ImageJ
GraphPad Prism
image J

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