Abstract
Whole-genome sequencing of penicillin-resistant Staphylococcus arlettae strain SAN1670 from bovine mastitis milk revealed a novel β-lactamase operon consisting of the β-lactamase-encoding gene blaARL, the antirepressor-encoding gene blaR1ARL, and the repressor-encoding gene blaIARL. The functionality of blaARL was demonstrated by gene expression in Staphylococcus aureus. The blaARL operon was chromosomally located in SAN1670 and present in 10 additional unrelated strains, suggesting intrinsic penicillin resistance in S. arlettae. Furthermore, a GenBank search revealed more unique potential β-lactamases in Staphylococcus species. IMPORTANCE Penicillins are an important group of antibiotics used to treat various types of infections caused by Gram-positive bacteria. So far, the blaZ gene was the only known β-lactamase gene in staphylococci. However, other putative β-lactamases were identified, and one of them was shown to be a novel functional β-lactamase encoded by blaARL in Staphylococcus arlettae, further limiting treatment options.
References
Jun 1, 1990·Molecular Microbiology·S J Rowland, K G Dyke
Oct 20, 1983·Nature·B N KreiswirthR P Novick
May 1, 1993·Antimicrobial Agents and Chemotherapy·C J Hackbarth, H F Chambers
Dec 15, 2000·Journal of Bacteriology·J R FitzgeraldC J Smyth
Mar 10, 2001·Science·H Z ZhangH F Chambers
May 3, 2003·The Journal of Clinical Investigation·Franklin D Lowy
Aug 12, 2004·Nucleic Acids Research·Lei ZhengJean-Louis Reymond
Mar 2, 2005·Veterinary Microbiology·Janaína dos Santos NascimentoMaria do Carmo de Freire Bastos
Feb 2, 2006·The Journal of Antimicrobial Chemotherapy·John Elmerdahl OlsenFrank Møller Aarestrup
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·George A Jacoby
Sep 29, 2006·Journal of Clinical Microbiology·Christina SchnellmannVincent Perreten
Oct 5, 2011·Antimicrobial Agents and Chemotherapy·Hsiao-Jan ChenLee-Jene Teng
Nov 15, 2011·Applied and Environmental Microbiology·Isabelle Verdier-MetzMarie-Christine Montel
Mar 3, 2012·G3 : Genes - Genomes - Genetics·Julia E S ShearerAnne O Summers
May 1, 2012·Bioinformatics·Matthew KearseAlexei Drummond
Nov 13, 2012·Journal of Bacteriology·Vasudevan DinakaranJeyaprakash Rajendhran
Nov 20, 2012·Nucleic Acids Research·Christian J A SigristIoannis Xenarios
Jul 6, 2013·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Karen Bush
Oct 11, 2013·Journal of Clinical Microbiology·J PiresL Peixe
Jun 6, 2014·The Veterinary Record·N De BriyneS P Borriello
Mar 10, 2015·Applied and Environmental Microbiology·Sybille Schwendener, Vincent Perreten
Apr 14, 2016·Annals of Clinical Microbiology and Antimicrobials·Ali KassimGunturu Revathi
Oct 21, 2016·Polish Journal of Veterinary Sciences·I KlimieneM Ruzauskas
Citations
Nov 9, 2019·Microbiology Resource Announcements·Hiroki YuKei Kitahara
Mar 6, 2018·Frontiers in Microbiology·Diego B NobregaHerman W Barkema
May 8, 2018·Journal of Dairy Science·Diego B NobregaHerman W Barkema
Nov 23, 2019·Microorganisms·Anna LavecchiaAntonio Placido
May 4, 2021·Frontiers in Veterinary Science·Jeroen De BuckHerman W Barkema
Jul 12, 2018·Microbiology Spectrum·Stefan SchwarzJianzhong Shen
Jul 31, 2021·BioMed Research International·Fikirte LemmaTadesse Eguale
Sep 9, 2021·Archives of Microbiology·Sukanya AcharyyaMalay Bhattacharya