Novel β-Lactamase blaARL in Staphylococcus arlettae

MSphere
Sabrina AndreisSybille Schwendener

Abstract

Whole-genome sequencing of penicillin-resistant Staphylococcus arlettae strain SAN1670 from bovine mastitis milk revealed a novel β-lactamase operon consisting of the β-lactamase-encoding gene blaARL, the antirepressor-encoding gene blaR1ARL, and the repressor-encoding gene blaIARL. The functionality of blaARL was demonstrated by gene expression in Staphylococcus aureus. The blaARL operon was chromosomally located in SAN1670 and present in 10 additional unrelated strains, suggesting intrinsic penicillin resistance in S. arlettae. Furthermore, a GenBank search revealed more unique potential β-lactamases in Staphylococcus species. IMPORTANCE Penicillins are an important group of antibiotics used to treat various types of infections caused by Gram-positive bacteria. So far, the blaZ gene was the only known β-lactamase gene in staphylococci. However, other putative β-lactamases were identified, and one of them was shown to be a novel functional β-lactamase encoded by blaARL in Staphylococcus arlettae, further limiting treatment options.

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Citations

Nov 9, 2019·Microbiology Resource Announcements·Hiroki YuKei Kitahara
May 4, 2021·Frontiers in Veterinary Science·Jeroen De BuckHerman W Barkema
Jul 12, 2018·Microbiology Spectrum·Stefan SchwarzJianzhong Shen

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Datasets Mentioned

BETA
KY363215
EGD-HP3
AVOQ01000023
X52734
KY363206

Methods Mentioned

BETA
PCR

Software Mentioned

Prosite
Geneious
TBLASTn
MEGA7
Clustal Omega

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