Novel α-Tubulin Mutations Conferring Resistance to Dinitroaniline Herbicides in Lolium rigidum

Frontiers in Plant Science
Zhizhan ChuStephen Powles

Abstract

The dinitroaniline herbicides (particularly trifluralin) have been globally used in many crops for selective grass weed control. Consequently, trifluralin resistance has been documented in several important crop weed species and has recently reached a level of concern in Australian Lolium rigidum populations. Here, we report novel mutations in the L. rigidum α-tubulin gene which confer resistance to trifluralin and other dinitroaniline herbicides. Nucleotide mutations at the highly conserved codon Arg-243 resulted in amino acid substitutions of Met or Lys. Rice calli transformed with the mutant 243-Met or 243-Lys α-tubulin genes were 4- to 8-fold more resistant to trifluralin and other dinitroaniline herbicides (e.g., ethalfluralin and pendimethalin) compared to calli transformed with the wild type α-tubulin gene from L. rigidum. Comprehensive modeling of molecular docking predicts that Arg-243 is close to the trifluralin binding site on the α-tubulin surface and that replacement of Arg-243 by Met/Lys-243 results in a spatial shift of the trifluralin binding domain, reduction of trifluralin-tubulin contacts, and unfavorable interactions. The major effect of these substitutions is a significant rise of free interaction energy be...Continue Reading

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Citations

Feb 10, 2019·The New Phytologist·Martin M Vila-AiubStephen B Powles
Oct 18, 2019·Pest Management Science·Yuichi MatsuzakiFukumatsu Iwahashi
Jun 26, 2020·Frontiers in Plant Science·Darren H TouchellThomas G Ranney
May 21, 2020·The Journal of Biological Chemistry·Todd A GainesFranck E Dayan
Oct 2, 2019·Plants·Brent P Murphy, Patrick J Tranel
Jun 30, 2019·The New Phytologist·Henrik Buschmann, Agnes Borchers
Dec 19, 2019·Plants·Eric L PattersonTodd A Gaines
Sep 30, 2020·Pest Management Science·Jinyi ChenStephen Powles
Dec 29, 2020·Journal of Integrative Plant Biology·Xiangqiang ZhanJose Ramon Botella
Mar 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Mariana GrancieriElvira Gonzalez de Mejia
Feb 9, 2021·Frontiers in Plant Science·Andréia K SuzukawaCaio A C G Brunharo
Apr 15, 2021·Molecular Plant-microbe Interactions : MPMI
Apr 13, 2021·Frontiers in Plant Science·Jinyi ChenStephen Powles
Jul 3, 2018·Journal of Agricultural and Food Chemistry·Jinyi ChenStephen Powles
Jul 10, 2018·Journal of Agricultural and Food Chemistry·Jingbo LiStephen Powles
Aug 28, 2021·Genes·Fidel González-Torralva, Jason K Norsworthy

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Datasets Mentioned

BETA
SRR1141083

Methods Mentioned

BETA
transgenic
PCR
phosphotransferase

Software Mentioned

Discovery
gmx
Studio Visualizer
PDB Viewer
mmpbsa
Swiss
S4MPLE
GROMACS
CHARMM
Swiss Param

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