Novel activation stimulus of chloride channels by potassium in human osteoblasts and human leukaemic T lymphocytes

The Journal of Physiology
M Steinert, Stephan Grissmer

Abstract

1. The whole-cell recording mode of the patch-clamp technique was used to study the effect of extracellular K+ and Rb+ on membrane currents in human osteoblasts, in a human osteoblast-like cell line, and in the Jurkat human leukaemic T cell line. 2. Increasing the extracellular concentration of K+ increased the membrane conductance of the cells in a concentration-dependent manner. This increase in membrane conductance was due to the activation of a Cl- conductance. Rb+ also induced this conductance, but conductance was less than half that seen in K+. 3. The Cl- channel blockers 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS) and 4-acetamido-4'-isothiocyanatostilbene 2,2'-disulphonic acid (SITS) blocked the K(+)-induced Cl- current in a voltage-dependent manner. The degree of blockade increased with membrane depolarization to a maximum level at 40 mV. At potentials above this value the block appeared to decrease. 4. Both tonicity and K+ were required for maximal activation of the Cl- conductance since the K(+)-induced Cl- conductance could be inhibited by hypertonic solutions and the activation of a volume-sensitive Cl- conductance by hypotonic solutions could be enhanced by extracellular K+. 5. We conclude that an ou...Continue Reading

Citations

Aug 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D Lovitch, M L Christianson
Aug 24, 1999·British Journal of Pharmacology·H Rauer, S Grissmer
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Susanna ZierlerHubert H Kerschbaum
Mar 15, 2003·American Journal of Physiology. Cell Physiology·Margot BräuerHeike Jäger
Sep 25, 2009·American Journal of Physiology. Cell Physiology·Neil C HenneyBronwen A J Evans
Oct 17, 2018·Frontiers in Neuroscience·Laura TheiTalitha L Kerrigan
Aug 4, 1998·The American Journal of Physiology·C Eder
Jan 13, 1998·Current Opinion in Biotechnology·M D Cahalan, K G Chandy

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