PMID: 8602352Feb 1, 1996Paper

Novel activities of human uracil DNA N-glycosylase for cytosine-derived products of oxidative DNA damage

Nucleic Acids Research
M DizdarogluH E Krokan

Abstract

Uracil DNA N-glycosylase is a repair enzyme that releases uracil from DNA. A major function of this enzyme is presumably to protect the genome from pre-mutagenic uracil resulting from deamination of cytosine in DNA. Here, we report that human uracil DNA N-glycosylase also recognizes three uracil derivatives that are generated as major products of cytosine in DNA by hydroxyl radical attack or other oxidative processes. DNA substrates were prepared by gamma-irradiation of DNA in aerated aqueous solution and incubated with human uracil DNA N-glycosylase, heat-inactivated enzyme or buffer. Ethanol-precipitated DNA and supernatant fractions were then separated. Supernatant fractions after derivatization, and pellets after hydrolysis and derivatization were analyzed by gas chromatography/isotope-dilution mass spectrometry. The results demonstrated that human uracil DNA N-glycosylase excised isodialuric acid, 5-hydroxyuracil and alloxan from DNA with apparent K(m) values of approximately 530, 450 and 660 nM, respectively. The excision of these uracil analogues is consistent with the recently described mechanism for recognition of uracil by human uracil DNA N-glycosylase [Mol,C.D., Arval,A.S., Slupphaug,G., Kavil,B., Alseth,I., Krokan,...Continue Reading

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Citations

Nov 26, 2003·Mutation Research·Geir SlupphaugHans E Krokan
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Sep 3, 2004·Mutation Research·Mark D EvansMarcus S Cooke
Aug 1, 1998·Mutation Research·B J GlassnerL D Samson
Dec 19, 2002·Free Radical Biology & Medicine·Marcus S CookeMark D Evans
Feb 9, 2006·Chemical Reviews·Patrick J O'Brien
Sep 2, 2004·The Journal of Biological Chemistry·Cheng-Yao ChenSamuel E Bennett
Jul 24, 2010·Antioxidants & Redox Signaling·David SvilarRobert W Sobol
Apr 15, 1996·Nucleic Acids Research·P Jaruga, M Dizdaroglu
Feb 1, 1997·Nucleic Acids Research·A KarakayaM Dizdaroglu
Mar 27, 2001·Nucleic Acids Research·M E Fárez-VidalD González-Pacanowska
Jan 14, 2005·Nucleic Acids Research·Grégory Eot-HoullierEvelyne Sage
Jan 13, 2006·Nucleic Acids Research·Lauren SeipleJames T Stivers
Feb 1, 2007·Nucleic Acids Research·Jason L ParsonsGrigory L Dianov
Jun 29, 2000·Annual Review of Biochemistry·A K McCulloughR S Lloyd
Apr 8, 2014·Mitochondrion·Meltem MuftuogluNadja C de Souza-Pinto
Aug 30, 2008·Critical Reviews in Biochemistry and Molecular Biology·Joke Baute, Anne Depicker
Jun 26, 2012·The International Journal of Biochemistry & Cell Biology·Víctor M Castillo-AcostaDolores González-Pacanowska
May 10, 2011·Progress in Neurobiology·Dennis Kjølhede JeppesenTinna Stevnsner
May 20, 2008·DNA Repair·Nadja C de Souza-PintoVilhelm A Bohr
Nov 1, 2015·Nucleic Acids Research·Nicholas C BauerPaul W Doetsch
Jun 26, 2007·Molecular Aspects of Medicine·Mirta M L SousaGeir Slupphaug
Jul 25, 2006·Experimental Cell Research·Lars HagenHans E Krokan
Apr 22, 2014·DNA Repair·Hans E KrokanGeir Slupphaug
Mar 31, 1999·Free Radical Research·C M GedikA R Collins
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Miral Dizdaroglu
Oct 23, 2016·Free Radical Biology & Medicine·Zhilei LiuHaiteng Deng
Jul 9, 2005·Biosensors & Bioelectronics·Lucilene Dornelles MelloLauro Tatsuo Kubota

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