Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex

Molecular Nutrition & Food Research
Keity Souza SantosFabio Fernandes Morato Castro

Abstract

Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients. The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome. Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and co...Continue Reading

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Aug 23, 2011·The Journal of Allergy and Clinical Immunology·Keity Souza SantosFabio Fernandes Morato Castro

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Citations

Mar 25, 2016·Journal of Occupational Health·Miaozong WuJian Liu
Jan 11, 2017·Journal of Agricultural and Food Chemistry·Yang YangGuang-Ming Liu
Jun 8, 2013·Molecular Nutrition & Food Research·Cláudia Regina Batista de Souza, Luiz Joaquim Castelo Branco Carvalho
Feb 18, 2014·Current Opinion in Clinical Nutrition and Metabolic Care·Wei Cai
Jun 20, 2020·Clinical and Translational Allergy·Claudia AlessandriAdriano Mari
Jun 30, 2019·Molecular Immunology·Ivana GiangriecoMaria Antonietta Ciardiello

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