PMID: 15383595Sep 24, 2004Paper

Novel and promiscuous CTL epitopes in conserved regions of Gag targeted by individuals with early subtype C HIV type 1 infection from southern Africa

The Journal of Immunology : Official Journal of the American Association of Immunologists
Agatha M MasemolaHIVNET 028 Study Team

Abstract

Characterization of optimal CTL epitopes in Gag can provide crucial information for evaluation of candidate vaccines in populations at the epicenter of the HIV-1 epidemic. We screened 38 individuals with recent subtype C HIV-1 infection using overlapping consensus C Gag peptides and hypothesized that unique HLA-restricting alleles in the southern African population would determine novel epitope identity. Seventy-four percent of individuals recognized at least one Gag peptide pool. Ten epitopic regions were identified across p17, p24, and p2p7p1p6, and greater than two-thirds of targeted regions were directed at: TGTEELRSLYNTVATLY (p17, 35%); GPKEPFRDYVDRFFKTLRAEQATQDV (p24, 19%); and RGGKLDKWEKIRLRPGGKKHYMLKHL (p17, 15%). After alignment of these epitopic regions with consensus M and a consensus subtype C sequence from the cohort, it was evident that the regions targeted were highly conserved. Fine epitope mapping revealed that five of nine identified optimal Gag epitopes were novel: HLVWASREL, LVWASRELERF, LYNTVATLY, PFRDYVDRFF, and TLRAEQATQD, and were restricted by unique HLA-Cw*08, HLA-A*30/B*57, HLA-A*29/B*44, and HLA-Cw*03 alleles, respectively. Notably, three of the mapped epitopes were restricted by more than one HLA al...Continue Reading

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Citations

Mar 29, 2008·PLoS Pathogens·Denis R ChoperaUNKNOWN CAPRISA 002 Study Team
Nov 6, 2008·Medical Microbiology and Immunology·Sanjay MendirattaVishnubhatla Sreenivas
Aug 30, 2008·The Journal of General Virology·Gerald K ChegeAnna-Lise Williamson
Jul 10, 2010·Journal of Biomedicine & Biotechnology·Giuseppina Li PiraFabrizio Manca
Mar 2, 2012·Journal of Virology·Jonathan M CarlsonDavid Heckerman
Aug 21, 2007·European Journal of Immunology·Nicole FrahmChristian Brander
Aug 17, 2011·The Journal of Infectious Diseases·Mopo RadebeThumbi Ndung'u
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isobella HoneybornePhilip J R Goulder
Jun 23, 2011·Immunogenetics·Xiangyu RaoCan Keşmir
Nov 17, 2006·Journal of Virology·Mayumi NakagawaAnna-Barbara Moscicki
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carina L PérezAnnika C Karlsson
Oct 28, 2009·The Journal of Experimental Medicine·Salim Abdool Karim
Nov 1, 2017·Scientific Reports·R Charlotte EcclestonNeil Dalchau
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