Novel antibiotic-free plasmid selection system based on complementation of host auxotrophy in the NAD de novo synthesis pathway.

Applied and Environmental Microbiology
Wei-Ren DongJian-Zhong Shao

Abstract

The use of antibiotic resistance genes in plasmids causes potential biosafety and clinical hazards, such as the possibility of horizontal spread of resistance genes or the rapid emergence of multidrug-resistant pathogens. This paper introduces a novel auxotrophy complementation system that allowed plasmids and host cells to be effectively selected and maintained without the use of antibiotics. An Escherichia coli strain carrying a defect in NAD de novo biosynthesis was constructed by knocking out the chromosomal quinolinic acid phosphoribosyltransferase (QAPRTase) gene. The resistance gene in the plasmids was replaced by the QAPRTase gene of E. coli or the mouse. As a result, only expression of the QAPRTase gene from plasmids can complement and rescue E. coli host cells in minimal medium. This is the first time that a vertebrate gene has been used to construct a nonantibiotic selection system, and it can be widely applied in DNA vaccine and gene therapy. As the QAPRTase gene is ubiquitous in species ranging from bacteria to mammals, the potential environmental biosafety problems caused by horizontal gene transfer can be eliminated.

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Citations

Jul 9, 2013·Trends in Biotechnology·Pedro H Oliveira, Juergen Mairhofer
Apr 21, 2011·Journal of Agricultural and Food Chemistry·Tien-Thanh NguyenClemens K Peterbauer
Sep 1, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gaëlle VandermeulenVéronique Préat
Apr 10, 2015·Pathogens·Charlotte MignonBettina Werle
Nov 21, 2015·Cell Death and Differentiation·T SharifP W K Lee
Apr 14, 2016·Biotechnology Progress·Desmond M SchofieldDarren N Nesbeth
Jan 18, 2015·Applied and Environmental Microbiology·Shicheng ChenEdward D Walker
May 7, 2021·Essays in Biochemistry·Aatir A TungekarLloyd W Ruddock
Oct 17, 2021·Biotechnology and Bioengineering·Dan GroffGang Yin

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