Novel application of complementary imaging techniques to examine in vivo glucose metabolism in the kidney.

American Journal of Physiology. Renal Physiology
Takashi HatoTimothy A Sutton

Abstract

The metabolic status of the kidney is a determinant of injury susceptibility and a measure of progression for many disease processes; however, noninvasive modalities to assess kidney metabolism are lacking. In this study, we employed positron emission tomography (PET) and intravital multiphoton microscopy (MPM) to assess cortical and proximal tubule glucose tracer uptake, respectively, following experimental perturbations of kidney metabolism. Applying dynamic image acquisition PET with 2-18fluoro-2-deoxyglucose (18F-FDG) and tracer kinetic modeling, we found that an intracellular compartment in the cortex of the kidney could be distinguished from the blood and urine compartments in animals. Given emerging literature that the tumor suppressor protein p53 is an important regulator of cellular metabolism, we demonstrated that PET imaging was able to discern a threefold increase in cortical 18F-FDG uptake following the pharmacological inhibition of p53 in animals. Intravital MPM with the fluorescent glucose analog 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG) provided increased resolution and corroborated these findings at the level of the proximal tubule. Extending our observation of p53 inhibition on pro...Continue Reading

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Citations

Jan 21, 2018·Journal of Diabetes Investigation·Susumu TakagiDaisuke Koya
Mar 3, 2017·Journal of the American Society of Nephrology : JASN·Takashi HatoPierre C Dagher
Jan 19, 2018·Current Protocols in Cytometry·Kenneth W DunnRuben M Sandoval
Dec 29, 2020·Frontiers in Pharmacology·Swayam Prakash SrivastavaJulie E Goodwin
Jan 3, 2018·Seminars in Nephrology·Georgina GyarmatiJános Peti-Peterdi
Mar 9, 2021·American Journal of Physiology. Renal Physiology·Kenneth W DunnPierre C Dagher

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